Angiogenic factor characterization and seminal vesicle lesions of transgenic adenocarcinoma mice prostate (TRAMP) in different life periods

• Grant number: 14/05364-3
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2014
• Effective date (End): May 31, 2015
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal researcher: Valéria Helena Alves Cagnon Quitete
• Grantee: Mariana Piccoli Gonçalves
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Angiogenesis is the process of get new vessels, which are fundamental to cancer set up. Tumor lesions in the seminal vesicle are often related to metastasis originated from prostate adenocarcinoma. However there are some doubts about the tumor origin in this organ, considering molecular aspects. Thus, the aim of this study is characterize the structure and molecular biology of the seminal vesicle by means of angiogenic factors (VEGF, endostatin, CD31) and Ki67 during different phases of tumor development, using seminal vesicle from transgenic adenocarcinoma of mouse prostate (TRAMP). The three experimental groups will use TRAMP mice in different ages: 8, 12 and 22 week old (n=6 per group) and three control group will use FVB mice in different ages: 8, 12 and 22 week old (n=6 per group). The seminal vesicle samples will be submitted to light microscopy analysis (Hematoxylin and Eosin and Masson' Tricomic). Also, imunuhistochemistry analyses will be carried out to VEGF, endostatin, KI67 and CD31. The proliferative índex and the microvessel density will be used, analyzing the CD31 and KI67.