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Analysis of mass spectrometry in the malignant transformation of the pleomorphic adenoma. correlation of data with gene expression and exome

Grant number: 19/06809-2
Support type:Scholarships in Brazil - Master
Effective date (Start): May 01, 2019
Effective date (End): July 31, 2020
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal researcher:Fernanda Viviane Mariano Brum Corrêa
Grantee:Reydson Alcides de Lima Souza
Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:15/07304-0 - Study of genetic and metabolic changes of pleomorphic adenoma and carcinoma ex pleomorphic adenoma by exome, gene expression and immunohistochemistry, AP.JP

Abstract

Proteomic analysis is defined as being the set of analytical methodologies employed to characterize, qualitatively and quantitatively, a proteome. It is an interdisciplinary area of science, which mainly aggregates chemistry, biology and computer science. Mass spectrometry (MS) separates and analyzes mixtures of molecules, or molecular fragments, based on their mass and charge. The use of these technologies has helped to understand the pathogenesis of certain lesions. Pleomorphic adenoma (PA) is the most common salivary gland neoplasm. Although it presents a benign nature, a possible complication of AP is the malignant transformation in Carcinoma Ex-Adenoma Pleomorphic (CXAP), being an aggressive neoplasm with metastatizing character and that can evolve to death. Considering that the etiopathogenic events of CXAP have not yet been elucidated, the objective of this study is to analyze by mass spectrometry the malignant transformation of PA. Given that etiopathogenic events of the CXAP have not yet been elucidated, the aim of this study is to analyze the malignant transformation of AP and to correlate it with gene expression results (study in development) using mass spectrometry. The work is based on laser microdissection of the tumor area of interest with subsequent protein extraction and analysis by mass spectrometry of the extracted product. We hope to correlate these data with gene expression and exome and believe that we can find viable markers for diagnosis and target therapy.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, ERISON SANTANA; NORMANDO, ANA GABRIELA COSTA; SCARINI, JOAO FIGUEIRA; CRESCENCIO, LIVIA RAMALHO; DE LIMA-SOUZA, REYDSON ALCIDES; MARIANO, FERNANDA VIVIANE; LEME, ADRIANA FRANCO PAES. Diagnostic and prognostic value of miRNAs on salivary gland tumors: a systematic review and meta-analysis. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, . (19/09692-9, 19/06809-2, 19/09419-0, 18/19922-9)
LIMA-SOUZA, REYDSON ALCIDES DE; SCARINI, JOAO FIGUEIRA; EGAL, ERIKA SAID ABU; CRESCENCIO, LIVIA RAMALHO; COSTA, JEZREEL CORRE DA; SILVA, MARIO FERNANDEZ SOBRAL; TINCANI, ALFIO JOSE; GONDAK, ROGERIO OLIVEIRA; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Secretory carcinoma ex pleomorphic adenoma of the submandibular gland: An immunohistochemical study. Oral Oncology, v. 120, . (19/06809-2, 15/07304-0, 19/09419-0)
DE LIMA-SOUZA, REYDSON ALCIDES; RODRIGUES, NATALIA DE MAGALHAES; SCARINI, JOAO FIGUEIRA; SOBRAL SILVA, MARIO FERNANDEZ; TINCANI, ALFIO JOSE; ABU EGAL, ERIKA SAID; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Metabolic alterations in carcinoma ex pleomorphic adenoma development of lacrimal glands. INTERNATIONAL OPHTHALMOLOGY, . (19/06809-2, 19/10788-0, 15/07304-0, 19/09419-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SOUZA, Reydson Alcides de Lima. Proteomic analysis based on mass spectrometry in the malignant transformation of Pleomorphic Adenoma. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.