Analysis of mass spectrometry in the malignant transformation of the pleomorphic adenoma. correlation of data with gene expression and exome

• Grant number: 19/06809-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2019
• End date: July 31, 2020
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Fernanda Viviane Mariano Brum Corrêa
• Grantee: Reydson Alcides de Lima Souza
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 15/07304-0 - Study of genetic and metabolic changes of pleomorphic adenoma and carcinoma ex pleomorphic adenoma by exome, gene expression and immunohistochemistry, AP.JP

Abstract

Proteomic analysis is defined as being the set of analytical methodologies employed to characterize, qualitatively and quantitatively, a proteome. It is an interdisciplinary area of science, which mainly aggregates chemistry, biology and computer science. Mass spectrometry (MS) separates and analyzes mixtures of molecules, or molecular fragments, based on their mass and charge. The use of these technologies has helped to understand the pathogenesis of certain lesions. Pleomorphic adenoma (PA) is the most common salivary gland neoplasm. Although it presents a benign nature, a possible complication of AP is the malignant transformation in Carcinoma Ex-Adenoma Pleomorphic (CXAP), being an aggressive neoplasm with metastatizing character and that can evolve to death. Considering that the etiopathogenic events of CXAP have not yet been elucidated, the objective of this study is to analyze by mass spectrometry the malignant transformation of PA. Given that etiopathogenic events of the CXAP have not yet been elucidated, the aim of this study is to analyze the malignant transformation of AP and to correlate it with gene expression results (study in development) using mass spectrometry. The work is based on laser microdissection of the tumor area of interest with subsequent protein extraction and analysis by mass spectrometry of the extracted product. We hope to correlate these data with gene expression and exome and believe that we can find viable markers for diagnosis and target therapy.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, ERISON SANTANA; NORMANDO, ANA GABRIELA COSTA; SCARINI, JOAO FIGUEIRA; CRESCENCIO, LIVIA RAMALHO; DE LIMA-SOUZA, REYDSON ALCIDES; MARIANO, FERNANDA VIVIANE; LEME, ADRIANA FRANCO PAES. Diagnostic and prognostic value of miRNAs on salivary gland tumors: a systematic review and meta-analysis. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, MAR 2021. (19/09692-9, 19/06809-2, 19/09419-0, 18/19922-9)

LIMA-SOUZA, REYDSON ALCIDES DE; SCARINI, JOAO FIGUEIRA; EGAL, ERIKA SAID ABU; CRESCENCIO, LIVIA RAMALHO; COSTA, JEZREEL CORRE DA; SILVA, MARIO FERNANDEZ SOBRAL; TINCANI, ALFIO JOSE; GONDAK, ROGERIO OLIVEIRA; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Secretory carcinoma ex pleomorphic adenoma of the submandibular gland: An immunohistochemical study. Oral Oncology, v. 120, SEP 2021. (19/06809-2, 15/07304-0, 19/09419-0)

SCARINI, JOAO FIGUEIRA; DE LIMA-SOUZA, REYDSON ALCIDES; LAVAREZE, LUCCAS; EMERICK, CAROLINA; MIYAOK, RAFAELLA DINI; SCHIAVON KINASZ, LETICIA RAYSA; MAUNSELL, REBECCA; ABU EGAL, ERIKA SAID; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Oral Teratoma with Hairy Polyp-Like Features: A Brief Report of a Rare Presentation. INTERNATIONAL JOURNAL OF SURGICAL PATHOLOGY, v. N/A, p. 4-pg., 2022-11-03. (21/12567-1, 15/07304-0, 19/09419-0, 19/06809-2)

DE LIMA-SOUZA, REYDSON ALCIDES; RODRIGUES, NATALIA DE MAGALHAES; SCARINI, JOAO FIGUEIRA; SOBRAL SILVA, MARIO FERNANDEZ; TINCANI, ALFIO JOSE; ABU EGAL, ERIKA SAID; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Metabolic alterations in carcinoma ex pleomorphic adenoma development of lacrimal glands. INTERNATIONAL OPHTHALMOLOGY, NOV 2021. (19/06809-2, 19/10788-0, 15/07304-0, 19/09419-0)

SCARINI, JOAO FIGURIRA; ALMEIDA MARIZ, BRUNO AUGESTO LINHARES; DE LIMA-SOUZA, REYDSON ALCIDES; LAVAREZE, LUCCAS; EMERICK, CAROLINA; TINCANI, ALFIO JOSE; ABU EGAL, ERIKA SAID; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVANE. Spindle cell carcinoma of the lip: An immunohistochemical study of a challenging case. Oral Oncology, v. 125, p. 3-pg., 2022-02-01. (19/06809-2, 15/07304-0, 19/09419-0, 18/24715-2)

ABU EGAL, ERIKA SAID; SCARINI, JOAO FIGUEIRA; DE LIMA-SOUZA, REYDSON ALCIDES; LAVAREZE, LUCCAS; FERNANDES, PATRICIA MARIA; EMERICK, CAROLINA; GONCALVES, MAYARA TREVIZOL; HELMS, MY N.; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Tumor microenvironment in salivary gland carcinomas: An orchestrated state of chaos. Oral Oncology, v. 127, p. 11-pg., 2022-02-18. (17/00831-0, 19/09419-0, 17/13167-1, 19/06809-2, 15/07304-0)

LAVAREZE, LUCCAS; SCARINI, JO AO FIGUEIRA; DE LIMA-SOUZA, REYDSON ALCIDES; EMERICK, CAROLINA; DE SA, RAISA SALES; AQUINO, IARA GONCALVES; FERNANDES, PATRICIA MARIA; GONCALVES, MAYARA TREVIZOL; HELMS, MY N.; ALTEMANI, ALBINA; et al. Salivary gland cancer in the setting of tumor microenvironment: Translational routes for therapy. CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, v. 171, p. 10-pg., 2022-03-01. (19/06809-2, 15/07304-0, 19/09419-0)

SCARINI, JOAO FIGUEIRA; LAVAREZE, LUCCAS; DE LIMA-SOUZA, REYDSON ALCIDES; EMERICK, CAROLINA; GONCALVES, MAYARA TREVIZOL; FIGUEIREDO-MACIEL, TAYNA; VIEIRA, GUSTAVO DE SOUZA; KIMURA, TALITA DE CARVALHO; DE SA, RAISA SALES; AQUINO, IARA GONCALVES; et al. Head and neck squamous cell carcinoma: Exploring frontiers of combinatorial approaches with tyrosine kinase inhibitors and immune checkpoint therapy. CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, v. 180, p. 14-pg., 2022-12-01. (15/07304-0, 21/12567-1, 19/09419-0, 19/06809-2)

LAVAREZE, LUCCAS; SCARINI, JOAO FIGUEIRA; LIMA-SOUZA, REYDSON ALCIDES DE; EGAL, ERIKA SAID ABU; TETZNER, DIEGO; LEITE, AMANDA ALMEIDA; VARGAS, PABLO AGUSTIN; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Granulomatous gingival enlargement in a patient with an unrecognized HIV infection. JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, v. 154, n. 7, p. 5-pg., 2023-07-01. (15/07304-0, 19/06809-2, 19/09419-0)

DOS SANTOS, ERISON SANTANA; NORMANDO, ANA GABRIELA COSTA; SCARINI, JOAO FIGUEIRA; CRESCENCIO, LIVIA RAMALHO; DE LIMA-SOUZA, REYDSON ALCIDES; MARIANO, FERNANDA VIVIANE; LEME, ADRIANA FRANCO PAES. Diagnostic and prognostic value of miRNAs on salivary gland tumors: a systematic review and meta-analysis. ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG, v. 25, n. 4, p. 12-pg., 2021-03-05. (18/19922-9, 19/09692-9, 19/09419-0, 19/06809-2)

DE LIMA-SOUZA, REYDSON ALCIDES; SCARINI, JOAO FIGUEIRA; LAVAREZE, LUCCAS; EMERICK, CAROLINA; DOS SANTOS, ERISON SANTANA; PAES LEME, ADRIANA FRANCO; ABU EGAL, ERIKA SAID; ALTEMANI, ALBINA; MARIANO, FERNANDA VIVIANE. Protein markers of primary salivary gland tumors: A systematic review of proteomic profiling studies. ARCHIVES OF ORAL BIOLOGY, v. 136, p. 13-pg., 2022-02-15. (19/06809-2, 18/19922-9, 19/09419-0, 15/07304-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Reydson Alcides de Lima. Proteomic analysis based on mass spectrometry in the malignant transformation of Pleomorphic Adenoma. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.