Proteomic analysis is defined as being the set of analytical methodologies employed to characterize, qualitatively and quantitatively, a proteome. It is an interdisciplinary area of science, which mainly aggregates chemistry, biology and computer science. Mass spectrometry (MS) separates and analyzes mixtures of molecules, or molecular fragments, based on their mass and charge. The use of these technologies has helped to understand the pathogenesis of certain lesions. Pleomorphic adenoma (PA) is the most common salivary gland neoplasm. Although it presents a benign nature, a possible complication of AP is the malignant transformation in Carcinoma Ex-Adenoma Pleomorphic (CXAP), being an aggressive neoplasm with metastatizing character and that can evolve to death. Considering that the etiopathogenic events of CXAP have not yet been elucidated, the objective of this study is to analyze by mass spectrometry the malignant transformation of PA. Given that etiopathogenic events of the CXAP have not yet been elucidated, the aim of this study is to analyze the malignant transformation of AP and to correlate it with gene expression results (study in development) using mass spectrometry. The work is based on laser microdissection of the tumor area of interest with subsequent protein extraction and analysis by mass spectrometry of the extracted product. We hope to correlate these data with gene expression and exome and believe that we can find viable markers for diagnosis and target therapy.
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