Cellular vacuolation and mitochondrial-associated factors induced by Clostridium perfringens epsilon toxin detected using acoustic flow cytometry

Autor(es)

Ferrarezi, Marina C. ^[1] ; Curci, Vera C. L. M. ^[2] ; Cardoso, Tereza C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, Dept Apoio Prod & Saude Anim, BR-16050680 Sao Paulo - Brazil

^[2] APTA, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anaerobe; v. 24, p. 55-59, DEC 2013.
• Citações Web of Science: 5

Resumo

Epsilon toxin (ETX) produced by Clostridium perfringens types B and D is a potent toxin that is responsible for fatal enterotoxaemia. In vitro, ETX, which is considered as a pore-forming toxin, forms a heptamer in Madin-Darby canine kidney (MOCK) cell membranes, which is considered to be a pre-pore stage. After binding of the ETX, vacuoles inside cell cytoplasm are produced. ETX causes decreased levels of essential coenzymes required for host cell energy. Here, we optimized and applied acoustic flow cytometry analysis in order to gain further insight into ETX-pathogenesis. Using acoustic flow cytometer analysis, which considered highly sensitive, ETX-exposed MOCK cells revealed mitochondrial membrane decreases followed by 25.48% and 45.45% of the exposed cells expressing the Bax and BCL-2 proteins at a pre-pore stage, respectively. These results together with high cytotoxicity and visualization of cell vacuoles, demonstrates that acoustic flow cytometry analysis potentially represents an effective tool to study ETX pathogenesis. (C) 2013 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 09/17635-3 - Estudo epidemiológico, anátomo-patológico e molecular da meningoencefalite não supurativa ocasionada pelo herpesvírus tipo 5
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/50782-7 - Susceptibilidade de células embrionárias de perus (mesenquimais respiratórias e digestivas) à infecção in vitro pelo coronavírus tipo III
• Beneficiário: Tereza Cristina Cardoso da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular