CHARACTERIZATION OF FRESHWATER BENTHIC BIOFILM-FORMING HYDROCORYNE (CYANOBACTERIA) ISOLATES FROM ANTARCTICA

Autor(es)

Genuario, Diego Bonaldo ^[1] ; Correa, Debora Machado ^[2] ; Komarek, Jiri ^{[3, 4]} ; Fiore, Marli Fatima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Sao Paulo - Brazil

^[2] Univ Fed Vicosa, Dept Bot, BR-36570000 Vicosa, MG - Brazil

^[3] Acad Sci Czech Republic, Inst Bot, CZ-37982 Trebon - Czech Republic

^[4] Univ South Bohemia, Fac Sci, CZ-37005 Ceske Budejovice - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYCOLOGY; v. 49, n. 6, p. 1142-1153, DEC 2013.
• Citações Web of Science: 12

Resumo

The aims of this work were to study cyanobacterial isolates resembling the genus Hydrocoryne using a combination of morphology and phylogeny of 16S rRNA and nifH sequences and to investigate genes involved in cyanotoxin and protease inhibitor production. Four new cyanobacterial strains, isolated from biofilm samples collected from King George Island, Antarctica, were studied. In terms of morphology, these new strains share traits similar to true Anabaena morphotypes (benthic ones), whereas phylogenetic analysis of their 16S rRNA gene sequences grouped them with the sequence of the type species Hydrocoryne spongiosa (H. Schwabe ex Bornet and Flahault 1886-1888), but not with sequences of the type species from the genus Anabaena. This cluster is the sister group of Anabaena morphotypes isolated only from the Gulf of Finland. In addition, this cluster is related to two other clusters formed by sequences of Anabaena isolated from different sites. Partial nifH genes were sequenced from two strains and the phylogenetic tree revealed that the Antarctic nifH sequences clustered with sequences from Anabaena. Furthermore, two strains were tested, using PCR with specific primers, for the presence of genes involved in cyanotoxins (microcystin and saxitoxin) and protease inhibitor (aeruginosin, and cyanopeptolin). Only cyanopeptolin was amplified using PCR. These four Hydrocoryne strains are the first to be isolated and sequenced from Antarctica, which improves our knowledge on this poorly defined cyanobacterial genus. (AU)

• Processo FAPESP: 10/16328-7 - Sequenciamento do genoma da cianobactéria Microcystis aeruginosa SPC777
• Beneficiário: Marli de Fátima Fiore
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/00321-3 - Diversidade e atividade funcional de cianobactérias do Arquipélago Shetlands do Sul, Continente Antártico
• Beneficiário: Diego Bonaldo Genuário
• Modalidade de apoio: Bolsas no Brasil - Doutorado