Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

CHARACTERIZATION OF FRESHWATER BENTHIC BIOFILM-FORMING HYDROCORYNE (CYANOBACTERIA) ISOLATES FROM ANTARCTICA

Full text
Author(s):
Genuario, Diego Bonaldo [1] ; Correa, Debora Machado [2] ; Komarek, Jiri [3, 4] ; Fiore, Marli Fatima [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13400970 Sao Paulo - Brazil
[2] Univ Fed Vicosa, Dept Bot, BR-36570000 Vicosa, MG - Brazil
[3] Acad Sci Czech Republic, Inst Bot, CZ-37982 Trebon - Czech Republic
[4] Univ South Bohemia, Fac Sci, CZ-37005 Ceske Budejovice - Czech Republic
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF PHYCOLOGY; v. 49, n. 6, p. 1142-1153, DEC 2013.
Web of Science Citations: 12
Abstract

The aims of this work were to study cyanobacterial isolates resembling the genus Hydrocoryne using a combination of morphology and phylogeny of 16S rRNA and nifH sequences and to investigate genes involved in cyanotoxin and protease inhibitor production. Four new cyanobacterial strains, isolated from biofilm samples collected from King George Island, Antarctica, were studied. In terms of morphology, these new strains share traits similar to true Anabaena morphotypes (benthic ones), whereas phylogenetic analysis of their 16S rRNA gene sequences grouped them with the sequence of the type species Hydrocoryne spongiosa (H. Schwabe ex Bornet and Flahault 1886-1888), but not with sequences of the type species from the genus Anabaena. This cluster is the sister group of Anabaena morphotypes isolated only from the Gulf of Finland. In addition, this cluster is related to two other clusters formed by sequences of Anabaena isolated from different sites. Partial nifH genes were sequenced from two strains and the phylogenetic tree revealed that the Antarctic nifH sequences clustered with sequences from Anabaena. Furthermore, two strains were tested, using PCR with specific primers, for the presence of genes involved in cyanotoxins (microcystin and saxitoxin) and protease inhibitor (aeruginosin, and cyanopeptolin). Only cyanopeptolin was amplified using PCR. These four Hydrocoryne strains are the first to be isolated and sequenced from Antarctica, which improves our knowledge on this poorly defined cyanobacterial genus. (AU)

FAPESP's process: 10/16328-7 - Genome sequencing of the cyanobacterium Microcystis aeruginosa SPC777
Grantee:Marli de Fátima Fiore
Support Opportunities: Regular Research Grants
FAPESP's process: 10/00321-3 - Diversity and functional activity of cyanobacteria isolated from South Shetlands Archipelago, Antarctic Continent
Grantee:Diego Bonaldo Genuário
Support Opportunities: Scholarships in Brazil - Doctorate