Tridimensional ultrastructure and glycolipid pattern studies of Trypanosoma dionisii

Autor(es)

de Castro Oliveira, Miriam Pires ^[1] ; Prata Ramos, Thiago Cesar ^[1] ; Pinheiro, Adriana Maria V. N. ^[1] ; Bertini, Silvio ^[2] ; Takahashi, Helio Kiyoshi ^[2] ; Straus, Anita Hilda ^[2] ; Haapalainen, Edna Freymuller ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biol Estrutural & Func, BR-04023900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Bioquim, BR-04023900 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Ctr Microscopia Eletron, BR-04023900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 128, n. 3, p. 548-556, DEC 2013.
• Citações Web of Science: 3

Resumo

Trypanosoma (Schizotrypanum) dionisii is a non-pathogenic bat trypanosome closely related to Trypanosoma cruzi, the etiological agent of Chaga's disease. Both kinetoplastids present similar morphological stages and are able to infect mammalian cells in culture. In the present study we examined 3D ultrastructure aspects of the two species by serial sectioning epimastigote and trypomastigote forms, and identified common carbohydrate epitopes expressed in T. dionisii, T. cruzi and Leishmania major. A major difference in 3D morphology was that T. dionisii epimastigote forms present larger multivesicular structures, restricted to the parasite posterior region. These structures could be related to T. cruzi reservosomes and are also rich in cruzipain, the major cysteine-proteinase of T. cruzi. We analyzed the reactivity of two monoclonal antibodies: MEST-1 directed to galactofuranose residues of glycolipids purified from Paracoccidioides brasiliensis, and BST-1 directed to glycolipids purified from T. cruzi epimastigotes. Both antibodies were reactive with T. dionisii epimastigotes by indirect immunofluorescense, but we noted differences in the location and intensity of the epitopes, when compared to T. cruzi. In summary, despite similar features in cellular structure and life cycle of T. dionisii and T. cruzi, we observed a unique morphological characteristic in T. dionisii that deserves to be explored. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/07005-4 - Microdomínios de membrana ricos em (glico) (esfingo) lipídeos e esteróis: organização, função e sinalização
• Beneficiário: Helio Kiyoshi Takahashi
• Modalidade de apoio: Auxílio à Pesquisa - Temático