Periodontal Treatment Downregulates Protease-Activated Receptor 2 in Human Gingival Crevicular Fluid Cells

Autor(es)

Euzebio Alves, Vanessa Tubero ^[1] ; Bueno da Silva, Henrique Aparecido ^[1] ; de Franca, Bruno Nunes ^[1] ; Eichler, Rosangela Santos ^[2] ; Saraiva, Luciana ^[1] ; Catelli de Carvalho, Maria Helena ^[2] ; Holzhausen, Marinella ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Infection and Immunity; v. 81, n. 12, p. 4399-4407, DEC 2013.
• Citações Web of Science: 12

Resumo

Protease-activated receptor 2 (PAR2) is implicated in the pathogenesis of chronic inflammatory diseases, including periodontitis; it can be activated by gingipain and produced by Porphyromonas gingivalis and by neutrophil protease 3 (P3). PAR2 activation plays a relevant role in inflammatory processes by inducing the release of important inflammatory mediators associated with periodontal breakdown. The effects of periodontal treatment on PAR2 expression and its association with levels of proinflammatory mediators and activating proteases were investigated in chronic periodontitis patients. Positive staining for PAR2 was observed in gingival crevicular fluid cells and was reflective of tissue destruction. Overexpression of PAR2 was positively associated with inflammatory clinical parameters and with the levels of interleukin-6 (IL-6), IL-8, tumor necrosis factor alpha, matrix metalloprotease 2 (MMP-2), MMP-8, hepatocyte growth factor, and vascular endothelial growth factor. Elevated levels of gingipain and P3 and decreased levels of dentilisin and the protease inhibitors secretory leukocyte protease inhibitor and elafin were also associated with PAR2 overexpression. Healthy periodontal sites from individuals with chronic periodontitis showed diminished expression of PAR2 mRNA and the PAR2 protein (P < 0.05). Furthermore, periodontal treatment resulted in decreased PAR2 expression and correlated with decreased expression of inflammatory mediators and activating proteases. We concluded that periodontal treatment resulted in decreased levels of proteases and that proinflammatory mediators are associated with decreased PAR2 expression, suggesting that PAR2 expression is influenced by the presence of periodontal infection and is not a constitutive characteristic favoring periodontal inflammation. (AU)

• Processo FAPESP: 10/16605-0 - Efeito da terapia periodontal sobre a expressão de receptor ativado por protease do tipo 2 (PAR-2) em pacientes com periodontite crônica.
• Beneficiário: Marinella Holzhausen Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular