Isolation and characterization of microsatellite markers in Rhaphiodon vulpinus (Cynodontidae, Characiformes) and their cross-amplification in other Cynodontinae species

Autor(es)

Garcez, Riviane ^[1] ; Francoso, Elaine ^[1] ; Sforca, Danilo A. ^[2] ; Souza, Anete P. ^[2] ; Almeida-Toledo, Lurdes F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508900 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, CBMEG, BR-13083875 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS RESOURCES; v. 5, n. 4, p. 1175-1177, DEC 2013.
• Citações Web of Science: 0

Resumo

Eleven microsatellite markers were developed for Rhaphiodon vulpinus, a widely distributed fish in the Amazon region. Recently, the Amazon fishes are being threatened by the construction of several dams, therefore genetic investigations are essential to further evaluate the impact of these dams on native species. Two R. vulpinus populations were analyzed and 3-20 alleles were found, with observed and expected heterozygosities ranging between 0.24 and 0.90 and between 0.22 and 0.90, respectively. After Bonferroni correction, no significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium were detected. These microsatellite loci were cross-amplified in others six Cynodontinae species and at least five loci demonstrated to be useful. (AU)

• Processo FAPESP: 10/52421-1 - Análises citogenetico-moleculares, filogenéticas e moleculares em espécies da ictiofauna neotropical
• Beneficiário: Lurdes Foresti de Almeida-Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/51279-7 - Novos estudos sobre a caracterizacao e evolucao de especies da ictiofauna neotropical: uma abordagem citogenetica e genetico-molecular
• Beneficiário: Lurdes Foresti de Almeida-Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular