Comparative Proteomic Analysis of the Aging Soleus and Extensor Digitorum Longus Rat Muscles Using TMT Labeling and Mass Spectrometry

Autor(es):Mostrar menos -

Chaves, Daniela F. S. ^[1] ; Carvalho, Paulo C. ^[2] ; Lima, Diogo B. ^[2] ; Nicastro, Humberto ^[1] ; Lorenzeti, Fabio M. ^[1] ; Siqueira-Filho, Mario ^[3] ; Hirabara, Sandro M. ^{[3, 4]} ; Alves, Paulo H. M. ^[5] ; Moresco, James J. ^[6] ; Yates, III, John R. ^[7] ; Lancha, Jr., Antonio H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sports, Lab Appl Nutr & Metab, BR-05508900 Sao Paulo - Brazil

^[2] Fiocruz MS, Carlos Chagas Inst, Lab Prote & Prot Engn, BR-81350010 Maringa, Parana - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[4] Univ Cruzeiro Sul, Inst Phys Act Sci & Sports, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo - Brazil

^[6] III, Scripps Res Inst, Dept Physiol Chem, San Diego, CA 92121 - USA

^[7] Yates, John R., III, Scripps Res Inst, Dept Physiol Chem, San Diego, CA 92121 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 12, n. 10, p. 4532-4546, OCT 2013.
• Citações Web of Science: 18

Resumo

Sarcopenia describes an age-related decline in skeletal muscle mass, strength, and function that ultimately impairs metabolism and leads to poor balance, frequent falling, limited mobility, and a reduction in quality of life. Here we investigate the pathogenesis of sarcopenia through a proteomic shotgun approach. In brief, we employed tandem mass tags to quantitate and compare the protein profiles obtained from young versus old rat slow-twitch type of muscle (soleus) and a fast-twitch type of muscle (extensor digitorum longus, EDL). Our results disclose 3452 and 1848 proteins identified from soleus and EDL muscles samples, of which 78 and 174 were found to be differentially expressed, respectively. In general, most of the proteins were structural related and involved in energy metabolism, oxidative stress, detoxification, or transport. Aging affected soleus and EDL muscles differently, and several proteins were regulated in opposite ways. For example, pyruvate kinase had its expression and activity different in both soleus and EDL muscles. We were able to verify with existing literature many of our differentially expressed proteins as candidate aging biomarkers and, most importantly, disclose several new candidate biomarkers such as the glioblastoma amplified sequence, zero beta-globin, and prolargin. (AU)

• Processo FAPESP: 09/52022-2 - Efeitos da suplementação com leucina nas vias de sinalização da síntese protéica em músculos sóleo e EDL de ratos jovens e idosos
• Beneficiário: Daniela Fojo Seixas Chaves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/07319-0 - Efeito da suplementação alimentar na melhora da densidade mineral óssea
• Beneficiário: Antonio Herbert Lancha Junior
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 10/10852-6 - Efeitos da suplementação com leucina nas vias de sinalização da síntese protéica em músculos sóleo e EDL de ratos jovens e idosos
• Beneficiário: Antonio Herbert Lancha Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular