Chaves, Daniela F. S.
Carvalho, Paulo C.
Lima, Diogo B.
Lorenzeti, Fabio M.
Hirabara, Sandro M.
Alves, Paulo H. M.
Moresco, James J.
Yates, III, John R.
Lancha, Jr., Antonio H.
Total Authors: 11
 Univ Sao Paulo, Sch Phys Educ & Sports, Lab Appl Nutr & Metab, BR-05508900 Sao Paulo - Brazil
 Fiocruz MS, Carlos Chagas Inst, Lab Prote & Prot Engn, BR-81350010 Maringa, Parana - Brazil
 Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil
 Univ Cruzeiro Sul, Inst Phys Act Sci & Sports, Sao Paulo - Brazil
 Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo - Brazil
 III, Scripps Res Inst, Dept Physiol Chem, San Diego, CA 92121 - USA
 Yates, John R., III, Scripps Res Inst, Dept Physiol Chem, San Diego, CA 92121 - USA
Total Affiliations: 7
JOURNAL OF PROTEOME RESEARCH;
Web of Science Citations:
Sarcopenia describes an age-related decline in skeletal muscle mass, strength, and function that ultimately impairs metabolism and leads to poor balance, frequent falling, limited mobility, and a reduction in quality of life. Here we investigate the pathogenesis of sarcopenia through a proteomic shotgun approach. In brief, we employed tandem mass tags to quantitate and compare the protein profiles obtained from young versus old rat slow-twitch type of muscle (soleus) and a fast-twitch type of muscle (extensor digitorum longus, EDL). Our results disclose 3452 and 1848 proteins identified from soleus and EDL muscles samples, of which 78 and 174 were found to be differentially expressed, respectively. In general, most of the proteins were structural related and involved in energy metabolism, oxidative stress, detoxification, or transport. Aging affected soleus and EDL muscles differently, and several proteins were regulated in opposite ways. For example, pyruvate kinase had its expression and activity different in both soleus and EDL muscles. We were able to verify with existing literature many of our differentially expressed proteins as candidate aging biomarkers and, most importantly, disclose several new candidate biomarkers such as the glioblastoma amplified sequence, zero beta-globin, and prolargin. (AU)