|Support type:||Scholarships in Brazil - Master|
|Effective date (Start):||August 01, 2013|
|Effective date (End):||March 31, 2015|
|Field of knowledge:||Health Sciences - Physical Education|
|Principal researcher:||Antonio Herbert Lancha Junior|
|Grantee:||Willian das Neves Silva|
|Home Institution:||Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil|
The objective of this study will be investigate the effects of strength training on survival and skeletal muscle of rats inoculated with Walker 256 tumor. Specifically, we are interested in investigating the effect of exercise on muscle structure and function of animals and the involvement of ubiquitin-proteasome proteolytic system and the involvement of protein synthesis via IGF-1/Akt/Mtor on muscle atrophy in rats with cachexia induced by cancer after strength training. Methods: Two experiments will be performed. The first will seek to verify that strength training increases survival of rats inoculated with walker 256 tumor, for this after the adaptation phase in training equipment, animals will be randomized to form 3 groups, namely : 1) controls (C, n = 5), 2) rats inoculated with Walker 256 tumor (W, n = 15) and 3) rats inoculated with Walker 256 tumor and subjected to strength training (WT, n = 15 ). Experimental groups will pass through the respective interventions until all the animals that developed tumor (WT and W groups) die. In the second experiment, after the adaptation phase, the animals will be randomized to compose 4 experimental groups, namely: 1) healthy rats - control (C, n = 10), 2) healthy mice subjected to strength training (CT, n = 10), 3) rats inoculated with Walker 256 tumor (W, n = 10) and 4) rats inoculated with Walker 256 tumor and subjected to strength training (WT, n = 10). Before tumor inoculation and 14 days after, all experimental groups will perform tests of strength and muscle function and maximal treadmill test. After 16 days of tumor inoculation, the animals will be euthanized and will be removed the muscles soleus and extensor digitorum longus (EDL) for histological and bio molecular analysis, including ex vivo muscle function, proteasome activity, gene expression of Atrogin, MuRF -1, and protein expression of IGF-1, AKT-1, mTOR and p70.