Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQO-induced potentially malignant oral lesions

Autor(es)

Barcessat, Ana Rita ^[1] ; Huang, Isaac ^[2] ; Rosin, Flavia Perillo ^[1] ; Pinto, Jr., Decio dos Santos ^[1] ; Zezell, Denise Maria ^[3] ; Correa, Luciana ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Gen Pathol Dept, BR-05508000 Sao Paulo - Brazil

^[3] IPEN CNEN SP, Nucl & Energy Res Inst, Dept Biophoton, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 126, p. 33-41, SEP 5 2013.
• Citações Web of Science: 7

Resumo

Fractionation can improve photodynamic therapy (PDT) efficacy for potentially malignant oral lesion treatment. The aim of this study was to demonstrate the apoptosis/proliferation index of oral keratinocytes after two sessions of topical 5-ALA-mediated PDT in 4-Nitroquinoline-1-oxide-induced potentially malignant oral lesion, and to suggest the ideal interval between PDT sessions. Immuno-histochemical tests for proliferating cell nuclear antigen and caspase-3, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed at 6 h, 24 h, 48 h, and 72 h time intervals after PDT. The number of positive cells showing caspase-3 expression was significantly higher, mainly at 6 h after PDT. In the first cycle of PDT, the highest frequency of positive cells for TUNEL was found at 24 h. At 72 h after PDT, proliferating cell nuclear antigen positive cells increased significantly, indicating that there was an epithelial response in direction towards DNA repair and cell proliferation at this time. Because cell proliferation increases and cell death index decreases at 72 h after PDT, it is recommended that the interval between the PDT sessions must not be longer than 2 days up to total lesion remission. (c) 2013 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/07437-0 - Análise da expressão de marcadores de morte celular em lesões potencialmente malignas induzidas em língua de ratos e tratadas com terapia fotodinâmica
• Beneficiário: Luciana Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular