Biochemical responses in armored catfish (Pterygoplichthys anisitsi) after short-term exposure to diesel oil, pure biodiesel and biodiesel blends

Autor(es)

Nogueira, Lilian ^[1] ; Humberto da Silva, Danilo Gruenig ^[1] ; Kikuchi Oliveira, Thiago Yukio ^[2] ; da Rosa, Joel Mauricio Correa ^[3] ; Felicio, Andreia Arantes ^[1] ; de Almeida, Eduardo Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista IBILCE UNESP, Dept Quim & Ciencias Ambientais, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14151140 Ribeirao Preto, SP - Brazil

^[3] Rockefeller Univ, Lab Invest Dermatol, New York, NY 10065 - USA

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 93, n. 2, p. 311-319, SEP 2013.
• Citações Web of Science: 11

Resumo

Biodiesel fuel is gradually replacing petroleum-based diesel oil use. Despite the biodiesel being considered friendlier to the environment, little is known about its effects in aquatic organisms. In this work we evaluated whether biodiesel exposure can affect oxidative stress parameters and biotransformation enzymes in armored catfish (Ptetygoplichthys anisitsi, Loricariidae), a South American endemic species. Thus, fish were exposed for 2 and 7 d to 0.01 mL L-1 and 0.1 mL L-1 of pure diesel, pure biodiesel (B100) and blends of diesel with 5% (B5) and 20% (B20) biodiesel. Lipid peroxidation (malondialdehyde) levels and the activities of the enzymes glutathione S-transferase, superoxide dismutase, catalase and glutathione peroxidase were measured in liver and gills. Also, DNA damage (8-oxo-7, 8-dihydro-2'-deoxyguanosine) levels in gills and 7-ethoxyresorufin-O-deethylase activity in liver were assessed. Pure diesel, B5 and B20 blends changed most of the enzymes tested and in some cases, B5 and B20 induced a higher enzyme activity than pure diesel. Antioxidant system activation in P. anisitsi was effective to counteract reactive oxygen species effects, since DNA damage and lipid peroxidation levels were maintained at basal levels after all treatments. However, fish gills exposed to B20 and B100 presented increased lipid peroxidation. Despite biodiesel being more biodegradable fuel that emits less greenhouse gases, the increased lipid peroxidation showed that biofuel and its blends also represent hazards to aquatic biota. (C) 2013 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/07449-5 - Estudo comparativo do estresse oxidativo entre tilápias (Oreochromis niloticus) e cascudos (Pterygoplichthys anisitsi) expostos a óleo diesel e a biodiesel
• Beneficiário: Lilian Nogueira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/58032-7 - Petrodiesel vs. biodiesel: a comparative study on their toxic effects in Nile tilapia and armoured catfishes
• Beneficiário: Eduardo Alves de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 06/03873-1 - Estudo de variações bioquímicas em tilápias, como biomarcadores de contaminação ambiental
• Beneficiário: Eduardo Alves de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores