Determination of Acetylmethionine in Injectable Solutions by Capillary Zone Electrophoresis

Autor(es):	Alexandre, Grazielle P. ^[1] ; Prado, Maria S. A. ^[1] ; Hackmann, Erika R. M. K. ^[1] ; Singh, Anil K. ^[1] ; Galdos, Angel A. G. ^[1] ; Santoro, Maria Ines R. M. ^[1] Número total de Autores: 6
Afiliação do(s) autor(es):	^[1] Univ Sao Paulo, Dept Pharm, Fac Pharmaceut Sci, Sao Paulo - Brazil Número total de Afiliações: 1
Tipo de documento:	Artigo Científico
Fonte:	Latin American Journal of Pharmacy; v. 32, n. 5, p. 657-662, JUN 2013.
Citações Web of Science:	0
Resumo
Acetylmethionine (AM) is a lipotropic drug used in several pharmaceutical formulations. The objective of this research is to develop and validate a capillary zone electrophoretic method for determination of AM in injectable solutions and its application in routine analysis for quality control. The capillary zone electrophoresis (CZE) determination was performed using an uncoated fused-silica capillary with 75 mu m i.d. and a total length of 402 cm (30 cm effective length) with a buffer solution containing 20 mmol/L sodium tetraborate, pH 9.2. The applied voltage was +25 kV and the sample was injected hydrodynamically at 0.5 psi for 5 s. The detection was made by ultraviolet (UV) absorption at 200 urn. The method is precise, selective, accurate and robust, and was successfully applied for quantitative determination of AM in injectable solutions. (AU)