Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine

Autor(es)

Oliveira, C. J. ^[1] ; Anatriello, E. ^[2] ; de Miranda-Santos, I. K. ^[2] ; Francischetti, I. M. ^[3] ; Sa-Nunes, A. ^[4] ; Ferreira, B. R. ^[5] ; Ribeiro, J. M. C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Triangulo Mineiro, Inst Biol & Nat Sci, BR-38025180 Uberaba, MG - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] NIAID, Lab Malaria & Vector Res, NIH, Bethesda, MD 20892 - USA

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Expt Immunol Lab, BR-05508900 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Sch Nursing, Dept Maternal & Child & Publ Hlth Nursing, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TICKS AND TICK-BORNE DISEASES; v. 4, n. 6, p. 469-477, 2013.
• Citações Web of Science: 31

Resumo

One dimensional gel electrophoresis was used to separate proteins from the saliva of Rhipicephalus sanguineus female ticks fed on rabbits. Gel slices were subjected to tryptic digestion and analyzed by reversed-phase HPLC followed by MS/MS analysis. The data were compared to a database of salivary proteins of the same tick and to the predicted proteins of the host. Saliva was obtained by either pilocarpine or dopamine stimulation of partially fed ticks. Electrophoretic separations of both yielded products that were identified by mass spectrometry, although the pilocarpine-derived sample was of much better quality. The majority of identified proteins were of rabbit origin, indicating the recycling of the host proteins in the tick saliva, including hemoglobin, albumin, haptoglobin, transferring, and a plasma serpin. The few proteins found that were previously associated with parasitism and blood feeding include 2 glycine-rich, cement-like proteins, 2 lipocalins, and a thyropin protease inhibitor. Among other of the 19 tick proteins identified, albeit with undefined roles, were SPARC and cyclophilin A. This catalog provides a resource that can be mined for secreted molecules that play a role in tick-host interactions. Published by Elsevier GmbH. (AU)

• Processo FAPESP: 07/50869-2 - Identificacao de antigenos protetores de carrapato do boi, rhipicephalus (boophilus) microplus, indutores de reacoes de hipersensibilidade tardia.
• Beneficiário: Elen Anatriello
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/53645-3 - Desenvolvimento de vacina anti-carrapatos: estudo do proteoma e imunoproteoma salivar do carrapato dos bovinos, Rhipicephalus, para identificação e caracterização de antígenos
• Beneficiário: Isabel Kinney Ferreira de Miranda Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/54985-4 - Efeito da saliva de carrapatos Rhipicephalus sanguineus sobre a expressão e ativação de receptores toll-like (TLRs)
• Beneficiário: Carlo José Freire de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado