Experimental host range of Citrus leprosis virus C (CiLV-C)

Autor(es)

Garita, Laura Cristina ^[1] ; Tassi, Aline Daniele ^[1] ; Calegario, Renata Faier ^[1] ; Freitas-Astua, Juliana ^{[2, 3]} ; Salaroli, Renato B. ^[1] ; Romao, Gerson O. ^[4] ; Kitajima, Elliot W. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

^[2] Embrapa Mandioca & Fruticultura, BR-43300000 Cruz Das Almas, BA - Brazil

^[3] Ctr APTA Citros Sylvio Moreira, BR-13590000 Cordeiropolis, SP - Brazil

^[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, Herbario ESA, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TROPICAL PLANT PATHOLOGY; v. 39, n. 1, p. 43-55, Fev. 2014.
• Citações Web of Science: 9

Resumo

Citrus leprosis (CL) is a serious threat to the citrus industry, especially for sweet oranges. For a long time, Citrus spp. were considered the only susceptible hosts. However, other plant species were also found either experimentally or naturally to be susceptible to Citrus leprosis virus C (CiLV-C). To assess the experimental host range of CiLV-C, a large number of plant species were inoculated with Brevipalpus phoenicis, viruliferous to CiLV-C, under experimental conditions. Out of the 140 tested species (43 families), 59 species (24 families) developed localized chlorotic and/or necrotic lesions upon inoculation of leaves with viruliferous mites, and 40 species (18 families) of them yielded positive results for CiLV-C detection in at least one of the following assays: ELISA, RT-PCR, transmission electron microscopy and immunfluorescence. For those that developed lesions and yielded negative results in CiLV-C detection assays, the results may be attributed to the small number of lesions and their necrotic state with very little viral material. The fact that a considerable number of plant species are susceptible to the virus after mite inoculation brings up implications for the epidemiology, quarantine and evolution of the citrus leprosis pathosystem. (AU)

• Processo FAPESP: 11/14496-2 - Avaliação da diversidade morfológica e genômica de diferentes populações de espécies de Brevipalpus (Acari: Tenuipalpidae) transmissores de vírus e de suas competências como vetor
• Beneficiário: Aline Daniele Tassi
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/52691-9 - Manejo da leprose dos citros
• Beneficiário: Elliot Watanabe Kitajima
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/53535-3 - Interação vírus/vetor/hospedeiro no patossistema leprose dos citros: análise por hibridização in situ de fluorescência e por imunofluorescência
• Beneficiário: Renata Faier Calegario
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado