Experimental host range of Citrus leprosis virus C (CiLV-C)

Autor(es)

Laura Cristina Garita ^[1] ; Aline Daniele Tassi ^[1] ; Renata Faier Calegario ^[1] ; Juliana Freitas-Astúa ^[4] ; Renato B. Salaroli ^[1] ; Gerson O. Romão ^[6] ; Elliot W. Kitajima ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

^[2] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Fitopatologia e Nematologia - Brasil

^[3] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz. Departamento de Ciências Biológicas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: TROPICAL PLANT PATHOLOGY; v. 39, n. 1, p. 43-55, 2014-01-31.

Resumo

Citrus leprosis (CL) is a serious threat to the citrus industry, especially for sweet oranges. For a long time, Citrus spp. were considered the only susceptible hosts. However, other plant species were also found either experimentally or naturally to be susceptible to Citrus leprosis virus C (CiLV-C). To assess the experimental host range of CiLV-C, a large number of plant species were inoculated with Brevipalpus phoenicis, viruliferous to CiLV-C, under experimental conditions. Out of the 140 tested species (43 families), 59 species (24 families) developed localized chlorotic and/or necrotic lesions upon inoculation of leaves with viruliferous mites, and 40 species (18 families) of them yielded positive results for CiLV-C detection in at least one of the following assays: ELISA, RT-PCR, transmission electron microscopy and immunfluorescence. For those that developed lesions and yielded negative results in CiLV-C detection assays, the results may be attributed to the small number of lesions and their necrotic state with very little viral material. The fact that a considerable number of plant species are susceptible to the virus after mite inoculation brings up implications for the epidemiology, quarantine and evolution of the citrus leprosis pathosystem. (AU)

• Processo FAPESP: 09/53535-3 - Interação vírus/vetor/hospedeiro no patossistema leprose dos citros: análise por hibridização in situ de fluorescência e por imunofluorescência
• Beneficiário: Renata Faier Calegario
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/52691-9 - Manejo da leprose dos citros
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/14496-2 - Avaliação da diversidade morfológica e genômica de diferentes populações de espécies de Brevipalpus (Acari: Tenuipalpidae) transmissores de vírus e de suas competências como vetor
• Beneficiário: Aline Daniele Tassi
• Modalidade de apoio: Bolsas no Brasil - Mestrado