Nanobiosensor for Diclofop Detection Based on Chemically Modified AFM Probes

Autor(es)

Bueno, Carolina Castro ^[1] ; Amarante, Adriano Moraes ^[1] ; Oliveira, Guedmiller S. ^[1] ; Deda, Daiana Kotra ^[1] ; Teschke, Omar ^[2] ; Franca, Eduardo de Faria ^[3] ; Leite, Fabio L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Nanoneurobiophys Res Grp, Dept Phys Chem & Math, BR-18052780 Sorocaba - Brazil

^[2] Univ Estadual Campinas, Inst Phys Gleb Wataghin, BR-13083970 Campinas, SP - Brazil

^[3] Univ Fed Uberlandia, Inst Chem, BR-18052780 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: IEEE SENSORS JOURNAL; v. 14, n. 5, p. 1467-1475, MAY 2014.
• Citações Web of Science: 11

Resumo

Highly sensitive and selective functional nanobio-breaksensors are being developed because they have significant applications in the sustenance and conservation of natural resources and can be used in projects to identify degraded and contaminated areas (of both soil and water) and as environmental quality indicators. In the present study, a nanobiosensor was developed based on using theoretical models (molecular docking and molecular dynamics simulations) based on biomimicry of the action mechanism of herbicides in plants coupled with atomic force microscopy (AFM) tools. The herbicide molecules were detected at very low concentrations using a unique sensor construction: the AFM probes and the substrate were chemically functionalized to favor covalent bonding and promote molecular flexibility, as well as to achieve reproducible and accurate results. Computational methods were used to determine the binding energies associated with the enzyme-herbicide interactions, which were compared with experimental results for adhesion forces. The theoretical results showed that the diclofop herbicide could be assembled and attached onto the mica substrate surface and the ACCase enzyme on the AFM probe without damaging the diclofop molecule. The experimental results showed that using a specific agrochemical target molecule was more efficient than using other nonspecific agrochemicals. On average, there was a 90% difference between the values of specific recognition (diclofop) and nonspecific recognition (imazaquin, metsulfuron, and glyphosate). This result validated the selectivity and specificity of the nanobiosensor. The first evidence of diclofop detection by the AFM probe sensors has been presented in this paper. (AU)

• Processo FAPESP: 09/09120-3 - Luciferases de coleópteros: evolução estrutural e funcional, e engenharia para fins biotecnológicos
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/05089-9 - Desenvolvimento de nanobiossensores usando a espectroscopia de força atômica: aplicação na detecção de pesticidas
• Beneficiário: Fabio de Lima Leite
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/09746-5 - Desenvolvimento de nanobiossensores utilizando técnicas computacionais avançadas
• Beneficiário: Guedmiller Souza de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado