Effects of tacrolimus and insulin in a liver regeneration model in growing animals with portal vein stenosis: immunohistochemical and molecular studies

Autor(es)

Backes, Ariane Nadia ^{[1, 2]} ; Aoun Tannuri, Ana Cristina ^{[1, 2]} ; Backes, Fabiane Neiva ^{[1, 2]} ; Rodrigues Queiroz, Amadeu Jose ^{[1, 2]} ; Mendonca Coelho, Maria Cecilia ^{[1, 2]} ; da Silva, Elenice Lima ^{[1, 2]} ; de Mello, Evandro Sobroza ^[3] ; Tannuri, Uenis ^{[4, 1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Div Pediat Surg, Pediat Liver Transplantat Unit, Sch Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Res Pediat Surg LIM 30, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Liver Funct Res Lab LIM 14, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PEDIATRIC SURGERY INTERNATIONAL; v. 30, n. 4, p. 423-429, APR 2014.
• Citações Web of Science: 1

Resumo

The aim of the present investigation was to describe a new model of liver regeneration in growing rats with reduced portal flow. In addition, it was studied whether tacrolimus and insulin could have any pro-regenerative effect under such conditions. Ninety-five rats were divided into five groups: Group 1 (sham), abdominal incision without intervention; Group 2, 70 % hepatectomy; Group 3, 70 % hepatectomy + PV stenosis; Group 4, 70 % hepatectomy + portal vein stenosis + insulin; and Group 5, 70 % hepatectomy + portal vein stenosis + tacrolimus. The remnant liver lobes were harvested for analyses. The liver weight decreased in the PV stenosis group and it increased with the use of insulin and tacrolimus. The mitotic activity was higher in the hepatectomy, insulin and tacrolimus groups and this parameter was reduced by portal stenosis. Levels of interleukin 6 (IL-6) were higher in the hepatectomy group compared to the sham and PV stenosis groups. The expression of IL-6 and Ki67 was significantly increased in the insulin and tacrolimus groups compared to the portal stenosis group. A highly reproducible model was standardized to study liver regeneration with portal blood inflow reduction in weaning rats. It was demonstrated that insulin or tacrolimus administration may partially reverse the harmful effects of PV stenosis. (AU)

• Processo FAPESP: 12/04122-0 - Modelos de regeneração hepática em animais jovens com estenose da veia porta ou ligadura da artéria hepática: estudos histológicos, imunoistoquímicos, moleculares: avaliação dos efeitos da insulina e do tacrolimus como agentes regenerativos
• Beneficiário: Ana Cristina Aoun Tannuri
• Linha de fomento: Auxílio à Pesquisa - Regular