Microbial composition of atherosclerotic plaques

Autor(es)

Calandrini, C. A. ^{[1, 2]} ; Ribeiro, A. C. ^[1] ; Gonnelli, A. C. ^[3] ; Ota-Tsuzuki, C. ^[1] ; Rangel, L. P. ^[4] ; Saba-Chujfi, E. ^[2] ; Mayer, M. P. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[2] Sao Leopoldo Mandic Univ, Dept Periodontol, Campinas, SP - Brazil

^[3] Beneficiencia Portuguesa Sao Paulo, Dept Cardiol, Sao Paulo - Brazil

^[4] Heart Hosp Para, Belem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 20, n. 3, p. e128-e134, APR 2014.
• Citações Web of Science: 25

Resumo

Objective The association of infections such as periodontitis with atherosclerotic diseases is well documented. In spite of the high diversity of the human oral microbiota, and its close contact with the circulatory system, few oral species were detected in atherosclerotic plaques. Thus, we attempted to evaluate the microbial diversity of atherosclerotic plaques from patients with different periodontal conditions, submitted to endarterectomy by a broad-range microbial method. Materials and Methods Patients indicated for aorta endarterectomy due to myocardial infarction were recruited for periodontal clinical examination. The microbial diversity of atherosclerotic plaques (n=35) was evaluated by sequence analysis of bacterial 16S rRNA libraries. Results Bacterial DNA was detected in 12 endarterectomy specimens (34.3%). Twenty-three bacterial species/phylotypes were identified. Proteobacteria and Firmicutes comprised 78.3% and 21.7% of the identified taxa, respectively. Fifteen (60.9%) phylotypes were reported as yet uncultivable or as yet uncharacterized species. Two uncultured phylotypes were previously detected in the human mouth. The periodontopathogen Aggregatibacter actinomycetemcomitans was detected in seven samples (20%), followed by Pseudomonas species. There was no association between periodontal parameters and detection of A.actinomycetemcomitans or other phylotypes in atherosclerotic plaques. Conclusion Our results suggest a role of the oral microbiota in the development of inflammation in atherogenesis, particularly of A.actinomycetemcomitans. (AU)

• Processo FAPESP: 06/59856-8 - Avaliação microbiológica por análise clonal de 16SrRNA da terapia endodôntica convencional e associada à irradiação do canal radicular com laser diodo
• Beneficiário: Adriana da Costa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado