Messenger RNAs in metaphase II oocytes correlate with successful embryo development to the blastocyst stage

Autor(es)

Biase, Fernando Henrique ^{[1, 2]} ; Everts, Robin Edward ^[2] ; Oliveira, Rosane ^[2] ; Freitas Santos-Biase, Weruska Karyna ^[3] ; Krempel Fonseca Merighe, Giovana ; Smith, Lawrence Charles ^[4] ; Martelli, Lucia ^[1] ; Lewin, Harris ^[2] ; Meirelles, Flavio Vieira ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049 Ribeirao Preto, SP - Brazil

^[2] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 - USA

^[3] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Ciencias Basicas, Pirassununga, SP - Brazil

^[4] Univ Montreal, Fac Med Vet, Ctr Rech Reprod Anim, St Hyacinthe, PQ J2S 7C6 - Canada

• Tipo de documento: Artigo Científico
• Fonte: ZYGOTE; v. 22, n. 1, p. 69-79, FEB 2014.
• Citações Web of Science: 8

Resumo

The mRNAs accumulated in oocytes provide support for embryo development until embryo genomic activation. We hypothesized that the maternal mRNA stock present in bovine oocytes is associated with embryo development until the blastocyst stage. To test our hypothesis, we analyzed the transcriptome of the oocyte and correlated the results with the embryo development. Our goal was to identify genes expressed in the oocyte that correlate with its ability to develop to the blastocyst stage. A fraction of oocyte cytoplasm was biopsied using micro-aspiration and stored for further expression analysis. Oocytes were activated chemically, cultured individually and classified according to their capacity to develop in vitro to the blastocyst stage. Microarray analysis was performed on mRNA extracted from the oocyte cytoplasm fractions and correlated with its ability to develop to the blastocyst stage (good quality oocyte) or arrest at the 8-16-cell stage (bad quality oocyte). The expression of 4320 annotated genes was detected in the fractions of cytoplasm that had been collected from oocytes matured in vitro. Gene ontology classification revealed that enriched gene expression of genes was associated with certain biological processes: RNA processing', translation' and mRNA metabolic process'. Genes that are important to the molecular functions of RNA binding' and translation factor activity, RNA binding' were also enriched in oocytes. We identified 29 genes with differential expression between the two groups of oocytes compared (good versus bad quality). The content of mRNAs expressed in metaphase II oocytes influences the activation of the embryonic genome and enables further develop to the blastocyst stage. (AU)

• Processo FAPESP: 06/57973-7 - Prospeccao de mensagens de competencia oocitaria em um modelo retrospectivo.
• Beneficiário: Fernando Henrique Biase
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado