| Texto completo | |
| Autor(es): |
Peres, Mariana Dadalto
[1]
;
Pelicao, Fabrcio Souza
[1, 2]
;
Caleffi, Bruno
[3]
;
De Martinis, Bruno Spinosa
[3]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14050140 Ribeirao Preto, SP - Brazil
[2] Serv Lab Med Legal, Dept Med Legal Vitoria, BR-29045402 Vitoria, ES - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14050140 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF ANALYTICAL TOXICOLOGY; v. 38, n. 1, p. 39-45, JAN-FEB 2014. |
| Citações Web of Science: | 7 |
| Resumo | |
A GCMS method for simultaneous analysis of cocaine (COC), amphetamines (AMPs), opiates, cannabinoids and their metabolites in vitreous humor (VH) was developed and fully validated. VH samples were extracted using solid phase extraction and injected into the GCMS, using a selected ion monitoring mode. Linearity ranged from 10 to 1000 ng/mL; the exception was anhydroecgonine methyl ester (AEME), for which linearity ranged from 10 to 750 ng/mL. Inter-assay imprecision lay from 1.2 to 10.0, intra-assay imprecision was 10.4 for all the analytes and accuracy ranged from 95.6 to 104.0. An limit of quantitation for all drugs was 10 ng/mL and recoveries ranged from 70.4 to 100.1 for basic and neutral compounds; the acid compounds had poor recoveryu40. The validated method was applied to 10 VH samples taken from individuals whose blood had screened positive for drugs of abuse. All the individuals screened positive for COC in the blood (seven samples) also had positive results in VH; COC concentration ranged from 30.81 to 283.97 ng/mL (mean 186.98 ng/mL) and benzoylecgonine concentration ranged from 11.47 to 460.98 ng/mL (mean 133.91 ng/mL). It was also noticed that, in five cases, cocaethylene was detected. AEME was also quantified in one case. The use of AMP detected by blood analysis was confirmed in the VH of one individual (24.31 ng/mL). However, samples taken from three individuals whose blood tested positive for carboxy-tetrahydrocannabinol presented negative results. The results demonstrated that VH is a suitable alternative biological sample to determine COC, AMPs, opiates and their metabolites. (AU) | |
| Processo FAPESP: | 10/05978-0 - Humor vítreo: uma alternativa para investigação de drogas de abuso post-mortem |
| Beneficiário: | Mariana Dadalto Peres |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 11/02210-7 - Humor vítreo: uma alternativa para investigação de drogas de abuso post-mortem |
| Beneficiário: | Bruno Spinosa de Martinis |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |