Electric Field-Assisted Solid Phase Extraction: Devices, Development and Application with a Cationic Model Compound

Autor(es)

Orlando, Ricardo Mathias ^[1] ; Rodrigues Rohwedder, Jarbas Jose ^[1] ; Rath, Susanne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Analyt Chem, Inst Chem, BR-13084971 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chromatographia; v. 77, n. 1-2, p. 133-143, JAN 2014.
• Citações Web of Science: 10

Resumo

This work describes novel devices for electric field-assisted solid phase extraction (E-SPE) and an application was carried out on the antimicrobial marbofloxacin (MAR). Conventional syringe-type SPE cartridges were easily adapted to receive two electrodes that were inserted and positioned below and above the sorbent. The adapted cartridges were coupled to a flow extraction system, which consisted of an electrophoresis power supply, a peristaltic pump and a homemade SPE manifold. These devices were used to apply electric fields during the extraction of MAR from fortified buffer and milk samples. The recovery of MAR was improved (2.3 times) or reduced (4.2 times) in comparison to conventional SPE when the top electrode was used as cathode (E-SPE(-/+)) or anode (E-SPE(+/-)), respectively. The results demonstrated that usual SPE cartridges can be easily and inexpensively adapted for applying an electric field in SPE. It was also concluded that the electric field applied in a usual SPE cartridge can be employed as a new suitable approach to enhance the extraction efficiency of ionic compounds in a complex sample matrix. (AU)

• Processo FAPESP: 08/03064-1 - Aplicação de potenciais elétricos em procedimentos de extração em fase sólida
• Beneficiário: Susanne Rath
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/07079-0 - Aplicação de potenciais elétricos em procedimentos de extração em fase sólida
• Beneficiário: Ricardo Mathias Orlando
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/11595-0 - Ampliação das fronteiras da e-spe®: desenvolvimento e avaliação de um sistema semi-automatizado
• Beneficiário: Ricardo Mathias Orlando
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado