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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enhancing effect of lysine combined with other compounds on cephamycin C production in Streptomyces clavuligerus

Texto completo
Autor(es):
Leite, Carla A. [1] ; Cavallieri, Andre P. [1] ; Araujo, Maria L. G. C. [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] UNESP, Sao Paulo State Univ, Dept Biochem & Technol Chem, BR-14800900 Araraquara, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: BMC Microbiology; v. 13, DEC 20 2013.
Citações Web of Science: 2
Resumo

Background: Lysine plays an important role in Streptomyces clavuligerus metabolism; it takes part in its catabolism, via cadaverine, and in its secondary metabolism, in which lysine is converted via 1-piperideine-6-carboxylate to alpha-aminoadipic acid, a beta-lactam antibiotic precursor. The role of lysine as an enhancer of cephamycin C production, when added to production medium at concentrations above 50 mmol l(-1), has already been reported in the literature, with some studies attributing a positive influence to multifunctional diamines, among other compounds. However, there is a lack of research on the combined effect of these compounds on antibiotic production. Results: Results from experimental design-based tests were used to conduct response surface-based optimization studies in order to investigate the synergistic effect of combining lysine with cadaverine, putrescine, 1,3-diaminopropane, or alpha-aminoadipic acid on cephamycin C volumetric production. Lysine combined with cadaverine influenced production positively, but only at low lysine concentrations. On the whole, higher putrescine concentrations (0.4 g l(-1)) affected negatively cephamycin C volumetric production. In comparison to culture media containing only lysine as additive, combinations of this amino acid with alpha-aminoadipic acid or 1,3-diaminopropane increased cephamycin C production by more than 100%. Conclusion: This study demonstrated that different combinations of lysine with diamines or lysine with alpha-aminoadipic acid engender significant differences with respect to antibiotic volumetric production, with emphasis on the benefits observed for lysine combined with alpha-aminoadipic acid or 1,3-diaminopropane. This increase is explained by mathematical models and demonstrated by means of bioreactor cultivations. Moreover, it is consistent with the positive influence of these compounds on lysine conversion to alpha-aminoadipic acid, a limiting step in cephamycin C production. (AU)

Processo FAPESP: 08/52819-5 - Adição de precursores em meio complexo solúvel para a produção de Cefamicina C por Streptomyces clavuligerus
Beneficiário:André Pastrelo Cavallieri
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 05/55079-4 - Produção e purificação de ácido clavulânico, cefamicina C e outros metabolitos bioativos de Streptomyces
Beneficiário:Carlos Osamu Hokka
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/02632-4 - Análise de fluxos metabólicos para a otimização in silico da produção de bioativos por Streptomyces clavuligerus
Beneficiário:Maria Lucia Gonsales da Costa Araujo
Linha de fomento: Auxílio à Pesquisa - Regular