| Texto completo | |
| Autor(es): |
Castro, Lilian dos Santos
[1]
;
Campos Antonieto, Amanda Cristina
[1]
;
Pedersoli, Wellington Ramos
[1]
;
Silva-Rocha, Rafael
[1]
;
Persinoti, Gabriela F.
[2]
;
Silva, Roberto Nascimento
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENE EXPRESSION PATTERNS; v. 14, n. 2, p. 88-95, MAR 2014. |
| Citações Web of Science: | 22 |
| Resumo | |
Trichoderma reesei is the most important fungus for the industrial production of enzymes to biomass deconstruction. Most of the genes encoding cellulases and hemicellulases are regulated by the transcription factors CRE1 and XYR1. In this work, the regulation of 22 genes of cellulases and xylanases by these transcription factors was investigated under three different carbon sources. Analysis of gene expression and enzymatic profiles of CMCase, beta-glucosidase, and xylanases showed different regulation that was depended of the carbon source in both Delta xyr1 and Delta cre1 mutants. In the presence of glucose, the majority of genes evaluated (82%) showed increased expression levels in the Delta cre1 mutant compared to the parental QM9414 strain. In the Delta xyr1 mutant, it was observed that expression of cellulase and xylanase genes was reduced compared to the parental QM9414 strain, when cultured in the presence of cellulose or sophorose. Interesting, in the presence of glucose, approximately 60% of the analyzed genes had increased expression in the Delta xyr1 mutant compared to parental strain. Furthermore, no correlation between gene expression and the number of putative binding sites of XYR1 and CRE1 to promoter region of cellulolytic and xylanolytic studied genes was observed. Therefore, these results demonstrated that the regulation of cellulase and xylanase by the transcription factors CRE1 and XYR1 is influenced by different carbon sources. (C) 2014 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 10/15683-8 - Estudos de sinalização celular e mecanismos de indução da formação de celulases pelo fungo Trichoderma reesei (Hypocrea jecorina) |
| Beneficiário: | Roberto do Nascimento Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores |