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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression pattern of cellulolytic and xylanolytic genes regulated by transcriptional factors XYR1 and CRE1 are affected by carbon source in Trichoderrna reesei

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Author(s):
Castro, Lilian dos Santos [1] ; Campos Antonieto, Amanda Cristina [1] ; Pedersoli, Wellington Ramos [1] ; Silva-Rocha, Rafael [1] ; Persinoti, Gabriela F. [2] ; Silva, Roberto Nascimento [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: GENE EXPRESSION PATTERNS; v. 14, n. 2, p. 88-95, MAR 2014.
Web of Science Citations: 22
Abstract

Trichoderma reesei is the most important fungus for the industrial production of enzymes to biomass deconstruction. Most of the genes encoding cellulases and hemicellulases are regulated by the transcription factors CRE1 and XYR1. In this work, the regulation of 22 genes of cellulases and xylanases by these transcription factors was investigated under three different carbon sources. Analysis of gene expression and enzymatic profiles of CMCase, beta-glucosidase, and xylanases showed different regulation that was depended of the carbon source in both Delta xyr1 and Delta cre1 mutants. In the presence of glucose, the majority of genes evaluated (82%) showed increased expression levels in the Delta cre1 mutant compared to the parental QM9414 strain. In the Delta xyr1 mutant, it was observed that expression of cellulase and xylanase genes was reduced compared to the parental QM9414 strain, when cultured in the presence of cellulose or sophorose. Interesting, in the presence of glucose, approximately 60% of the analyzed genes had increased expression in the Delta xyr1 mutant compared to parental strain. Furthermore, no correlation between gene expression and the number of putative binding sites of XYR1 and CRE1 to promoter region of cellulolytic and xylanolytic studied genes was observed. Therefore, these results demonstrated that the regulation of cellulase and xylanase by the transcription factors CRE1 and XYR1 is influenced by different carbon sources. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/15683-8 - Studies of cellular signaling and induction mechanisms of cellulases formation by the fungus Trichoderma reesei (Hypocrea jecorina)
Grantee:Roberto do Nascimento Silva
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants