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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice

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Autor(es):
Baldon, Estevam Jose [1] ; Marengo, Eliana Blini [2] ; de Franco, Marcelo [3] ; Starobinas, Nancy [3] ; Bueno, Valquiria [4] ; Sant'Anna, Osvaldo Augusto [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Imunoquim, BR-05530900 Sao Paulo - Brazil
[2] Hosp Israelita Albert Einstein, BR-05652000 Sao Paulo - Brazil
[3] Inst Butantan, Lab Imunogenet, BR-05530900 Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: IMMUNITY & AGEING; v. 11, MAR 26 2014.
Citações Web of Science: 1
Resumo

Background: Aging process may result in immune modifications that lead to disruption of innate and acquired immunity mechanisms that may induce chronic-degenerative events. The heat shock proteins (Hsp), phylogeneticaly conserved among organisms, present as main function the ability of folding and refolding proteins, but they also are associated with chronic-degenerative disorders. Here were evaluated the role of M. leprae native Hsp65 (WT) and its point-mutated (K(409)A) on survival and anti-DNA and anti-Hsp65 antibody production of aged genetically selected mice for high (H-III) and low (L-III) antibody production; data from 120- and 270-days old mice (named ``adult{''} or ``aged{''}, respectively) were compared. Results: WT Hsp65 administration induces reduction in the mean survival time of adult and aged female HIII mice, this effect being stronger in aged individuals. Surprisingly, the native protein administration increased the survival of aged female LIII when compared to K(409)A and control groups. No survival differences were observed in aged male mice after Hsp65 proteins inoculation. We observed increase in IgG1 anti-Hsp65 in WT and K(409)A aged HIII female mice groups and no marked changes in the anti-DNA (adult and aged HIII) and anti-Hsp65 IgG1 or IgG2a isotypes production in adult HIII female and aged male mice. LIII male mice presented increased anti-DNA and anti-Hsp65 IgG2a isotype production after WT or K(409)A injection, and LIII female groups showed no alterations. Conclusions: The results revealed that the WT Hsp65 interferes with survival of aged HIII female mice without involvement of a remarkable IgG1 and IgG2a anti-DNA and anti-Hsp65 antibodies production. The deleterious effects of Hsp65 on survival time in aged HIII female mice could be linked to a gender-effect and are in agreement with those previously reported in lupus-prone mice. (AU)

Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
Beneficiário:Hugo Aguirre Armelin
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs