A capillary electrophoresis/tandem mass spectrometry approach for the determination of monoalkyl carbonates

Autor(es)

do Lago, Claudimir Lucio ^[1] ; Mendona Francisco, Kelliton Jose ^[1] ; Daniel, Daniela ^[2] ; Rajh Vidal, Denis Tadeu ^[1] ; dos Santos, Vagner Bezerra ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, BR-05508000 Sao Paulo - Brazil

^[2] Agilent Technol Brasil, BR-06460040 Barueri, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: RSC ADVANCES; v. 4, n. 38, p. 19674-19679, 2014.
• Citações Web of Science: 4

Resumo

The hemiesters of carbonic acid, which include the monoalkyl carbonates (MACs), are a poorly-known class of species with potential interest for biological processes. Capillary electrophoresis (CE) with tandem mass spectrometry is herein introduced as a complementary technique to CE with capacitively coupled contactless conductivity detection ((CD)-D-4) for the study of MACs. Multiple reactions monitoring mode was used to improve sensitivity and selectivity, the loss of CO2 (44 u) at low collisional energy being the key feature of the MACs. To improve the control over the temperature -and consequently over the hydrolysis of the MACs during the electrophoretic migration - a thermostating case for the silica capillary was developed. Quantitation was possible by using the estimated concentration of MAC from the initial concentrations of the reagents and the equilibrium constant in the calibration procedure. The LOD for monoethyl carbonate was 0.2 mu mol L-1, which is ca. two orders of magnitude lower than the LOD obtained by CE-(CD)-D-4. Using a modified BGE for the separation of MACs, the LODs for mono-3-pentyl, mono-1-butyl, mono-2-propyl, and monoethyl carbonates were 0.2, 0.5, 2, and 1.3 mu mol L-1, respectively. (AU)

• Processo FAPESP: 11/02156-2 - Investigação do uso da eletroforese capilar com detecção condutométrica sem contato para a obtenção de parâmetros analíticos e físico-químicos para peroxicarbonato, perácidos e monoetil pirocarbonato.
• Beneficiário: Denis Tadeu Rajh Vidal
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/14993-1 - Caracterização e quantificação de monoalquil carbonatos em meio aquoso empregando dispositivos microfluídicos acoplado a um espectrômetro de massas
• Beneficiário: Vagner Bezerra dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/06642-1 - Formação e propriedades de adutos de hidróxi-compostos orgânicos com carbonato em meio aquoso e condições biológicas
• Beneficiário: Claudimir Lucio Do Lago
• Modalidade de apoio: Auxílio à Pesquisa - Regular