Nanobiosensors Exploiting Specific Interactions Between an Enzyme and Herbicides in Atomic Force Spectroscopy

Autor(es)

da Silva, Aline C. N. ^[1] ; Deda, Daiana K. ^[1] ; Bueno, Carolina C. ^[1] ; Moraes, Ariana S. ^[1] ; Da Roz, Alessandra L. ^[1] ; Yamaji, Fabio M. ^[1] ; Prado, Rogilene A. ^[2] ; Viviani, Vadim ^[2] ; Oliveira, Jr., Osvaldo N. ^[3] ; Leite, Fabio L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Phys Chem & Math, Lab Biochem & Biotechnol Bioluminescence, BR-18052780 Sorocaba, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Nanoscience and Nanotechnology; v. 14, n. 9, p. 6678-6684, SEP 2014.
• Citações Web of Science: 8

Resumo

The development of sensitive methodologies for detecting agrochemicals has become important in recent years due to the increasingly indiscriminate use of these substances. In this context, nanosensors based on atomic force microscopy (AFM) tips are useful because they provide higher sensitivity with operation at the nanometer scale. In this paper we exploit specific interactions between AFM tips functionalized with the enzyme acetolactate synthase (ALS) to detect the ALS-inhibitor herbicides metsulfuron-methyl and imazaquin. Using atomic force spectroscopy (AFS) we could measure the adhesion force between tip and substrate, which was considerably higher when the ALS-functionalized tip (nanobiosensor) was employed. The increase was approximately 250% and 160% for metsulfuron-methyl and imazaquin, respectively, in comparison to unfunctionalized probes. We estimated the specific enzyme-herbicide force by assuming that the measured force comprises an adhesion force according to the Johnson-Kendall-Roberts (JKR) model, the capillary force and the specific force. We show that the specific, biorecognition force plays a crucial role in the higher sensitivity of the nanobiosensor, thus opening the way for the design of similarly engineered tips for detecting herbicides and other analytes. (AU)

• Processo FAPESP: 10/04599-6 - Estudo de "Superfícies Inteligentes" para Desenvolvimento de Nanobiossensores
• Beneficiário: Aline Cristine Nanuh da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/09120-3 - Luciferases de coleópteros: evolução estrutural e funcional, e engenharia para fins biotecnológicos
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/05089-9 - Desenvolvimento de nanobiossensores usando a espectroscopia de força atômica: aplicação na detecção de pesticidas
• Beneficiário: Fabio de Lima Leite
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/00463-2 - Estudo de Materiais Biológicos usando a Microscopia de Força Atômica
• Beneficiário: Gabrielle de Almeida Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica