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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanobiosensors Exploiting Specific Interactions Between an Enzyme and Herbicides in Atomic Force Spectroscopy

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da Silva, Aline C. N. [1] ; Deda, Daiana K. [1] ; Bueno, Carolina C. [1] ; Moraes, Ariana S. [1] ; Da Roz, Alessandra L. [1] ; Yamaji, Fabio M. [1] ; Prado, Rogilene A. [2] ; Viviani, Vadim [2] ; Oliveira, Jr., Osvaldo N. [3] ; Leite, Fabio L. [1]
Total Authors: 10
[1] Univ Fed Sao Carlos, Dept Phys Chem & Math, Nanoneurobiophys Res Grp, BR-18052780 Sorocaba, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Phys Chem & Math, Lab Biochem & Biotechnol Bioluminescence, BR-18052780 Sorocaba, SP - Brazil
[3] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Nanoscience and Nanotechnology; v. 14, n. 9, p. 6678-6684, SEP 2014.
Web of Science Citations: 8

The development of sensitive methodologies for detecting agrochemicals has become important in recent years due to the increasingly indiscriminate use of these substances. In this context, nanosensors based on atomic force microscopy (AFM) tips are useful because they provide higher sensitivity with operation at the nanometer scale. In this paper we exploit specific interactions between AFM tips functionalized with the enzyme acetolactate synthase (ALS) to detect the ALS-inhibitor herbicides metsulfuron-methyl and imazaquin. Using atomic force spectroscopy (AFS) we could measure the adhesion force between tip and substrate, which was considerably higher when the ALS-functionalized tip (nanobiosensor) was employed. The increase was approximately 250% and 160% for metsulfuron-methyl and imazaquin, respectively, in comparison to unfunctionalized probes. We estimated the specific enzyme-herbicide force by assuming that the measured force comprises an adhesion force according to the Johnson-Kendall-Roberts (JKR) model, the capillary force and the specific force. We show that the specific, biorecognition force plays a crucial role in the higher sensitivity of the nanobiosensor, thus opening the way for the design of similarly engineered tips for detecting herbicides and other analytes. (AU)

FAPESP's process: 07/05089-9 - Development of nanobiosensors using atomic force spectroscopy: application for detecting pesticides
Grantee:Fabio de Lima Leite
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 09/09120-3 - Beetle luciferases: structural, functional evolution and enegineering for biotechnological purposes
Grantee:Vadim Viviani
Support type: Regular Research Grants
FAPESP's process: 10/04599-6 - Development of nanobiosensors based on intelligent surfaces
Grantee:Aline Cristine Nanuh da Silva
Support type: Scholarships in Brazil - Master