Reliability and validity of digital assessment of perifoveal capillary network measurement using high-resolution imaging

Autor(es)

Lopes de Faria, Jacqueline Mendonca ^[1] ; Duarte, Diego Andreazzi ^[1] ; Larico Chavez, Roger Fredy ^[2] ; Arthur, Angelica Moises ^[2] ; Arthur, Rangel ^[2] ; Iano, Yuzo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Res Dept, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Fac Elect & Computat Engineer, Lab Visual Commun, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BRITISH JOURNAL OF OPHTHALMOLOGY; v. 98, n. 6, p. 726-729, JUN 2014.
• Citações Web of Science: 9

Resumo

Background Assessment of the perifoveal capillary network (PCN) might indicate macular function and could reflect the systemic microcirculation. The quantification and reliability of this measurement is currently unknown. The aim of this study was to validate quantification of the PCN by a non-invasive technique from high-resolution retinal images. Methods Ten healthy volunteers were included in this validation study. At least 320 high-resolution retinal images were used for assessment of inter-and intraobserver reliability. Non-invasive capillary perfusion mapping was performed using a retinal function imager. After the images were enhanced and segmented, the reproducibility was verified by comparing the values of two independent examiners and of a single examiner at two different time points. Results The inter-observer concordance coefficients were highly significant for PCN (intraclass correlation coefficient (ICC)=0.901, 95% CI 0.655 to 0.975, p<0.001) and normalised PCN (ICC=0.727, 95% CI 0.262 to 0.923, p=0.004). The intra-observer measurements at two different time points were also highly concordant for PCN (ICC=0.879, 95% CI 0.598 to 0.968, p<0.001) and for normalised PCN (ICC=0.960, 95% CI 0.851 to 0.990, p<0.001). Conclusions The reliability of PCN measurement is reproducible and could be used as a new tool to quantify the capillary perfusion network of the macular area. (AU)

• Processo FAPESP: 09/52890-4 - Avaliação in vivo dos possíveis efeitos agudos de agentes antioxididantes na microcirculação da retina de pacientes diabéticos através de uma nova tecnologia computacional oftalmológica não invasiva: possíveis efeitos da ingestão do cacau e do chá verde na microcirculação retiniana
• Beneficiário: Jacqueline Mendonça Lopes de Faria
• Modalidade de apoio: Auxílio à Pesquisa - Regular