| Texto completo | |
| Autor(es): |
Kido, Larissa Akemi
[1]
;
Hetzl, Amanda Cia
[1]
;
Candido, Eduardo Marcelo
[1]
;
Montico, Fabio
[1]
;
Lorencini, Raisa Mistieri
[1]
;
Alves Cagnon, Valeria Helena
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083865 Campinas, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Life Sciences; v. 106, n. 1-2, p. 58-70, JUN 13 2014. |
| Citações Web of Science: | 4 |
| Resumo | |
Aims: The aim of this study was to evaluate the structural and molecular effects of antiangiogenic therapies and finasteride on the ventral prostate of senile mice. Main methods: 90 male FVB mice were divided into: Young (18 weeks old) and senile (52 weeks old) groups; finasteride group: finasteride (20 mg/kg); SU5416 group: SU5416 (6 mg/kg); TNP-470 group: TNP-470 (15 mg/kg,) and SU5416 + TNP-470 group: similar to the SU5416 and TNP-470 groups. After 21 days, prostate ventral lobes were collected for morphological, immunohistochemical and Western blotting analyses. Key findings: The results demonstrated atrophy, occasional proliferative lesions and inflammatory cells in the prostate during senescence, which were interrupted and/or blocked by treatment with antiangiogenic drugs and finasteride. Decreased AR and endostatin reactivities, and an increase for ER-alpha, ER-beta and VEGF, were seen in the senile group. Decreased VEGF and ER-a reactivities and increased ER-beta reactivity were verified in the finasteride, SU5416 groups and especially in SU5416 + TNP-470 group. The TNP-470 group showed reduced AR and ER-beta protein levels. Significance: The senescence favored the occurrence of structural and/or molecular alterations suggesting the onset of malignant lesions, due to the imbalance in the signaling between the epithelium and stroma. The SU5416 + TNP-470 treatment was more effective in maintaining the structural, hormonal and angiogenic factor balance in the prostate during senescence, highlighting the signaling of antiproliferation via ER-beta. (C) 2014 Elsevier Inc. All rights reserved. (AU) | |
| Processo FAPESP: | 12/03010-4 - Caracaterização molecular da próstata frente às terapias hormonais e antiangiogênicas em camundongos senis (FVB) e no modelo transgênicos (Tramp) para adenocarcinoma de próstata |
| Beneficiário: | Valéria Helena Alves Cagnon Quitete |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/03116-4 - Terapias antiangiogênicas, uso de finasterida e resposta hormonal na senilidade na próstata de camundongos |
| Beneficiário: | Larissa Akemi Kido de Barros |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |