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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)

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Autor(es):
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Silva, Duilio M. Z. de A. [1] ; Pansonato-Alves, Jose Carlos [1] ; Utsunomia, Ricardo [1] ; Araya-Jaime, Cristian [1] ; Ruiz-Ruano, Francisco J. [2] ; Daniel, Sandro Natal [3] ; Hashimoto, Diogo Teruo [4] ; Oliveira, Claudio [1] ; Camacho, Juan Pedro M. [2] ; Porto-Foresti, Fabio [3] ; Foresti, Fausto [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Dept Morfol, Inst Biociencias, Sao Paulo - Brazil
[2] Univ Granada, Dept Genet, Granada - Spain
[3] Univ Estadual Paulista, Dept Ciencias Biol, Fac Ciencias, Sao Paulo - Brazil
[4] Univ Estadual Paulista, CAUNESP, Sao Paulo - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 9, n. 4 APR 15 2014.
Citações Web of Science: 46
Resumo

Supernumerary (B) chromosomes have been shown to contain a wide variety of repetitive sequences. For this reason, fluorescent in situ hybridisation (FISH) is a useful tool for ascertaining the origin of these genomic elements, especially when combined with painting from microdissected B chromosomes. In order to investigate the origin of B chromosomes in the fish species Astyanax paranae, these two approaches were used along with PCR amplification of specific DNA sequences obtained from the B chromosomes and its comparison with those residing in the A chromosomes. Remarkably, chromosome painting with the one-arm metacentric B chromosome probe showed hybridization signals on entire B chromosome, while FISH mapping revealed the presence of H1 histone and 18S rDNA genes symmetrically placed in both arms of the B chromosome. These results support the hypothesis that the B chromosome of A. paranae is an isochromosome. Additionally, the chromosome pairs Nos. 2 or 23 are considered the possible B chromosome ancestors since both contain syntenic H1 and 18S rRNA sequences. The analysis of DNA sequence fragments of the histone and rRNA genes obtained from the microdissected B chromosomes showed high similarity with those obtained from 0B individuals, which supports the intraspecific origin of B chromosomes in A. paranae. Finally, the population hereby analysed showed a female-biased B chromosome presence suggesting that B chromosomes in this species could influence sex determinism. (AU)

Processo FAPESP: 11/16825-3 - ESTRUTURA MOLECULAR E CITOGENÉTICA DE CROMOSSOMOS B EM Astyanax paranae (CHARACIFORMES, CHARACIDAE)
Beneficiário:Duílio Mazzoni Zerbinato de Andrade Silva
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 10/17009-2 - Biodiversidade e relações filogenéticas dos gêneros Astyanax, Hemigrammus, Hyphessobrycon e Moenkhausia (Characiformes: Characidae)
Beneficiário:Claudio de Oliveira
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático