Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

Autor(es)

Elza F.T. Belo ^[1] ; Ligia M.C.C. Coutinho ^[2] ; Aline S. Ferraz ^[3] ; Elizabeth N. De Gaspari ^[4]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Institute - Brasil

^[2] Adolfo Lutz Institute - Brasil

^[3] Adolfo Lutz Institute - Brasil

^[4] Adolfo Lutz Institute - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Infectious Diseases; v. 8, n. 6, p. 407-418, 2004-12-00.

Resumo

A new monoclonal antibody (5F81A4P1.9), which is specific for subtype 9 antigen of meningococci, was studied. The antibodies were raised against a previously non-typable (NT) serogroup B strain from Brazilian patients and were found to react with the subtype antigen of prototype reference strains for subtype 9 (M982), as well as with those of homologous strains. The subtype 9 epitope was found in 6.8% of serogroup B strains among 602 strains of Neisseria meningitidis case isolates, including representative isolates from Brazilian states. Subtype P1.9 was predominantly related to serogroup B in Brazil among the isolates collected during the N. meningitidis epidemic in 1992. No significant differences were observed in the occurrence of subtype P1.9 among strains isolated from several Brazilian states. Fluorescence-activated cell-sorter analysis showed that 5F81A4 MAb recognized a 46 kDa protein on the surface of a homologous strain of N. meningitidis (B:4:P1.9). These results, in association with a bactericidal activity assay for 5F81A4, and with experimental passive protection in mice, demonstrated the importance of subtype 9 class 1 proteins of N meningitidis in Brazil. Serotyping is essential for the development of vaccination strategies. (AU)

• Processo FAPESP: 99/00557-6 - Imunização nasal em camundongos com antígenos de Neisseria meningitidis B: obtenção de anticorpos monoclonais e perfil da produção in vitro de interleucinas
• Beneficiário: Elizabeth Natal de Gaspari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 99/00638-6 - Imunização nasal em camundongos com antígenos de Neisseria meningitidis B: obtenção de anticorpos monoclonais e perfil da produção in vitro de interleucinas
• Beneficiário: Lígia Maria de Castro Carvalho Coutinho
• Modalidade de apoio: Bolsas no Brasil - Mestrado