Usefulness of a rapid immunometric assay for intraoperative parathyroid hormone measurements

Autor(es)

M.N. Ohe ^[1] ; R.O. Santos ^[2] ; I.S. Kunii ^[3] ; A.B. Carvalho ^[4] ; M. Abrahão ^[5] ; O. Cervantes ^[6] ; M. Lazaretti-Castro ^[7] ; J.G.H. Vieira ^[8]

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Endocrinologia

^[2] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Cirurgia de Cabeça e Pescoço - Brasil

^[3] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Endocrinologia

^[4] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Nefrologia

^[5] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Cirurgia de Cabeça e Pescoço - Brasil

^[6] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Cirurgia de Cabeça e Pescoço - Brasil

^[7] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Endocrinologia

^[8] Universidade Federal de São Paulo. Escola Paulista de Medicina. Disciplina de Endocrinologia

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 36, n. 6, p. 715-721, 2003-06-00.

Resumo

Intraoperative parathyroid hormone (IO-PTH) measurements have been proposed to improve operative success rates in primary, secondary and tertiary hyperparathyroidism (PHP, SHP and THP). Thirty-one patients requiring parathyroidectomy were evaluated retrospectively from June 2000 to January 2002. Sixteen had PHP, 7 SHP and 8 THP. Serum samples were taken at times 0 (before resection), 10, 20 and 30 min after resection of each abnormal parathyroid gland. Samples from 28 patients were frozen at -70ºC for subsequent tests, whereas samples from three patients were tested while surgery was being performed. IO-PTH was measured using the Elecsys immunochemiluminometric assay (Roche, Mannheim, Germany). The time necessary to perform the assay was 9 min. All samples had a second measurement taken by a conventional immunofluorimetric method. We considered as cured patients who presented normocalcemia in PHP and THP, and normal levels of PTH in SHP one month after surgery and who remained in this condition throughout the follow-up of 1 to 20 months. When rapid PTH assay was compared with a routine immunofluorimetric assay, excellent correlation was observed (r = 0.959, P < 0.0001). IO-PTH measurement showed a rapid average decline of 78.8% in PTH 10 min after adenoma resection in PHP and all patients were cured. SHP patients had an average IO-PTH decrease of 89% 30 min after total parathyroidectomy and cure was observed in 85.7%. THP showed an average IO-PTH decrease of 91.9%, and cure was obtained in 87.5% of patients. IO-PTH can be a useful tool that might improve the rate of successful treatment of PHP, SHP and THP. (AU)

• Processo FAPESP: 00/00843-8 - Aplicação de analisador baseado em citometria de fluxo visando a: 1) desenvolvimento de processo de baixo custo para análise simultânea de múltiplos parâmetros de interesse para diagnóstico e acompanhamento de pacientes portadores de patologias tiroideanas; 2) desenvolvimento de ensaio imunométrico rápido para dosagem do PTH intra-operatoriamente e seu emprego como auxílio à decisão em relação ao sucesso cirúrgico
• Beneficiário: José Gilberto Henriques Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Regular