Interspecific variation of the bacterial community structure in the phyllosphere of the three major plant components of mangrove forests

Autor(es)

Armando Cavalcante Franco Dias ^{[1, 2]} ; Rodrigo Gouveia Taketani ^[2] ; Fernando Dini Andreote ^[3] ; Danice Mazzer Luvizotto ^[3] ; João Luis da Silva ^[5] ; Rosely dos Santos Nascimento ^[2] ; Itamar Soares de Melo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba, SP - Brazil

^[2] Empresa Brasileira Pesquisa Agr, Lab Microbiol Ambiental, Jaguariuna, SP - Brazil

^[3] Univ Sao Paulo, Dept Ciencia Solo, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[4] Empresa Brasileira de Pesquisa Agropecuária. Laboratório de Microbiologia Ambiental - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 43, n. 2, p. 653-660, 2012-06-00.

Resumo

Mangrove forests encompass a group of trees species that inhabit the intertidal zones, where soil is characterized by the high salinity and low availability of oxygen. The phyllosphere of these trees represent the habitat provided on the aboveground parts of plants, supporting in a global scale, a large and complex microbial community. The structure of phyllosphere communities reflects immigration, survival and growth of microbial colonizers, which is influenced by numerous environmental factors in addition to leaf physical and chemical properties. Here, a combination of culture-base methods with PCR-DGGE was applied to test whether local or plant specific factors shape the bacterial community of the phyllosphere from three plant species (Avicenia shaueriana, Laguncularia racemosa and Rhizophora mangle), found in two mangroves. The number of bacteria in the phyllosphere of these plants varied between 3.62 x 10(4) in A. schaeriana and 6.26 x 10³ in R. mangle. The results obtained by PCR-DGGE and isolation approaches were congruent and demonstrated that each plant species harbor specific bacterial communities in their leaves surfaces. Moreover, the ordination of environmental factors (mangrove and plant species), by redundancy analysis (RDA), also indicated that the selection exerted by plant species is higher than mangrove location on bacterial communities at phyllosphere. (AU)

• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 10/50799-7 - Microrganismos envolvidos na ciclagem de nitrogenio na rizosfera de plantas nativas da caatinga.
• Beneficiário: Rodrigo Gouvêa Taketani
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado