Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

Autor(es):Mostrar menos -

Kossuga, Miriam H. ^[1] ; Romminger, Stelamar ^[1] ; Xavier, Camila ^[1] ; Milanetto, Marilia C. ^[1] ; do Valle, Milene Z. ^[1] ; Pimenta, Eli F. ^[1] ; Morais, Raquel P. ^[1] ; de Carvalho, Erica ^{[1, 2]} ; Mizuno, Carolina M. ^{[1, 2]} ; Coradello, Luis Fernando C. ^{[1, 2]} ; Barroso, Vinicius de M. ^{[1, 2]} ; Vacondio, Bruna ^{[1, 2]} ; Javaroti, Darci C. D. ^[2] ; Seleghim, Mirna H. R. ^[2] ; Cavalcanti, Bruno C. ^[3] ; Pessoa, Claudia ^[3] ; Moraes, Manoel O. ^[3] ; Lima, Bruna A. ^[4] ; Goncalves, Reginaldo ^[4] ; Bonugli-Santos, Rafaella C. ^[5] ; Sette, Lara D. ^[5] ; Berlinck, Roberto G. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Ecol & Biol Evolut, BR-13560 Sao Carlos, SP - Brazil

^[3] Univ Fed Ceara, Dept Fisiol & Farmacol, Fortaleza, Ceara - Brazil

^[4] Univ Estadual Campinas, Dept Diagnost Oral, Fac Odontol Piracicaba, BR-13081970 Campinas, SP - Brazil

^[5] Univ Estadual Paulista, Dept Bioquim & Microbiol, Rio Claro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 22, n. 2, p. 257-267, MAR-APR 2012.
• Citações Web of Science: 26

Resumo

In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K. (AU)

• Processo FAPESP: 10/50190-2 - Investigação do potencial biotecnológico e metabólico de organismos marinhos para processos de biorremediação e produção de substâncias com atividades anti-virais, anti-leishmania e anti-inflamatória (Biodiversidade Marinha)
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 05/60175-2 - Descoberta e desenvolvimento de potenciais agentes quimioterápicos a partir de invertebrados marinhos e de microrganismos associados
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático