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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

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Kossuga, Miriam H. [1] ; Romminger, Stelamar [1] ; Xavier, Camila [1] ; Milanetto, Marilia C. [1] ; do Valle, Milene Z. [1] ; Pimenta, Eli F. [1] ; Morais, Raquel P. [1] ; de Carvalho, Erica [1, 2] ; Mizuno, Carolina M. [1, 2] ; Coradello, Luis Fernando C. [1, 2] ; Barroso, Vinicius de M. [1, 2] ; Vacondio, Bruna [1, 2] ; Javaroti, Darci C. D. [2] ; Seleghim, Mirna H. R. [2] ; Cavalcanti, Bruno C. [3] ; Pessoa, Claudia [3] ; Moraes, Manoel O. [3] ; Lima, Bruna A. [4] ; Goncalves, Reginaldo [4] ; Bonugli-Santos, Rafaella C. [5] ; Sette, Lara D. [5] ; Berlinck, Roberto G. S. [1]
Total Authors: 22
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Ecol & Biol Evolut, BR-13560 Sao Carlos, SP - Brazil
[3] Univ Fed Ceara, Dept Fisiol & Farmacol, Fortaleza, Ceara - Brazil
[4] Univ Estadual Campinas, Dept Diagnost Oral, Fac Odontol Piracicaba, BR-13081970 Campinas, SP - Brazil
[5] Univ Estadual Paulista, Dept Bioquim & Microbiol, Rio Claro - Brazil
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 27

In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G) K. (AU)

FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
Grantee:Roberto Gomes de Souza Berlinck
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 05/60175-2 - Discovery and development of potential chemotherapeutic agents based on marine invertebrates and associated micro-organisms
Grantee:Roberto Gomes de Souza Berlinck
Support type: BIOTA-FAPESP Program - Thematic Grants