Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector

Autor(es)

de Lima Procopio, Rudi Emerson ^{[1, 2]} ; Araujo, Welington Luiz ^{[3, 1]} ; Andreote, Fernando Dini ^[1] ; Azevedo, Joao Lucio ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed 4, Sao Paulo - Brazil

^[3] Univ Mogi das Cruzes, Mogi Das Cruzes, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 34, n. 1, p. 103-109, 2011.
• Citações Web of Science: 5

Resumo

A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle vector pLGM1 was constructed by combining the pPAGA plasmid with pGFPmut3.0 (which harbors a gene encoding green fluorescent protein, GFP), and the resulting construct was used to over-express GFP in E. coli and P. agglomerans cells. GFP production was used to monitor the colonization of strain EGE6gfp in various plant tissues by fluorescence microscopy. Analysis of EGE6gfp colonization showed that 14 days after inoculation, the strain occupied the inner tissue of Eucalyptus grandis roots, preferentially colonizing the xylem vessels of the host plants. (AU)

• Processo FAPESP: 00/07053-2 - Caracterização da biodiversidade bacteriana endofítica de Eucalyptus spp. e avaliação do seu potencial biotecnológico
• Beneficiário: Rudi Emerson de Lima Procópio
• Modalidade de apoio: Bolsas no Brasil - Mestrado