Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from Sao Paulo State, Brazil

Autor(es)

Porfida Ferreira, Thais Sebastiana ^[1] ; Moreno, Andrea Micke ^[2] ; de Almeida, Renata Rodrigues ^[1] ; Gomes, Cleise Ribeiro ^[1] ; Sena de Gobbi, Debora Dirani ^[1] ; Nogueira de Lima Filsner, Pedro Henrique ^[1] ; Moreno, Marina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FMVZ, Dept Med Vet Prevent & Sande Anim VPS, Lab Sanidade Suina & Virol, Programa Posgrad Epide, BR-05508270 Sao Paulo - Brazil

^[2] Univ Sao Paulo, FMVZ, VPS, Lab Sanidade Suina & Virol, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Ciência Rural; v. 42, n. 8, p. 1450-1456, AUG 2012.
• Citações Web of Science: 3

Resumo

Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes. using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE). Clostridium perfringens isolation frequencies in carcasses and finishing pig intestines were of 58.8% in both types of samples. According to the polymerase chain reaction assay, only, alfa toxin was detected, being all isolates also negative to enterotoxin and beta2 toxin. Through PFGE technique, the strains were characterized in 35 pulsotypes. In only one pulsotype, the isolate from carcass sample was grouped with fecal isolate of the same animal, suggesting that the risk of cross-contamination was low. Despite the high prevalence of C. perfringens in swine carcasses from the slaughterhouses assessed, the risk of food poisoning to Brazilian pork consumers is low, since all strains were negative to cpe-gene, codifying enterotoxin. (AU)

• Processo FAPESP: 03/01645-3 - Caracterização genotípica de amostras de Clostridium perfringens provenientes de suínos através da PCR-ribotipagem, do polimorfismo do comprimento de fragmentos amplificados (AFLP) e da eletroforese em gel de campo pulsado (PFGE)
• Beneficiário: Andrea Micke Moreno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 04/04024-2 - Caracterizacao genotipica de isolados de clostridium perfringens provenientes de suinos, atraves da pcr-ribotipagem.
• Beneficiário: Renata Rodrigues de Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 04/04023-6 - Caracterizacao genotipica de amostras de clostridium perfringens de origem suina atraves de eletroforese em campo pulsado (pfge).
• Beneficiário: Thaís Sebastiana Porfida Ferreira
• Modalidade de apoio: Bolsas no Brasil - Mestrado