Nephrocalcinosis induced by hyperoxaluria in rats

Autor(es)

Cunha, Natalia Baraldi ^[1] ; Kawano, Paulo Roberto ^[2] ; Padovani, Carlos Roberto ^[3] ; Lima, Flavio de Oliveira ^[4] ; Bernardes, Suene ^[5] ; Magalhaes, Eloa Siqueira ^[6] ; Petean Amaro, Carmen Regina ^[7] ; Amaro, Joao Luiz ^[8]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP. Urol Expt Lab

^[2] Sao Paulo State Univ UNESP. Urol Expt Lab

^[3] Sao Paulo State Univ UNESP. Urol Expt Lab

^[4] Sao Paulo State Univ UNESP. Urol Expt Lab

^[5] Sao Paulo State Univ UNESP. Urol Expt Lab

^[6] Sao Paulo State Univ UNESP. Urol Expt Lab

^[7] Sao Paulo State Univ UNESP. Urol Expt Lab

^[8] Sao Paulo State Univ UNESP. Urol Expt Lab

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 28, n. 7, p. 499-504, JUL 2013.
• Citações Web of Science: 1

Resumo

PURPOSE: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. METHODS: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). RESULTS: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. CONCLUSION: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days. (AU)

• Processo FAPESP: 11/11699-0 - Nefrolitíase induzida pela hiperoxalúria: modelo experimental em ratos
• Beneficiário: João Luiz Amaro
• Modalidade de apoio: Auxílio à Pesquisa - Regular