The Selection Exerted by Oil Contamination on Mangrove Fungal Communities

Autor(es)

Fasanella, Cristiane Cipola ^[1] ; Franco Dias, Armando Cavalcante ^[2] ; Rigonato, Janaina ^[3] ; Fiore, Marli de Fatima ^[4] ; Soares, Jr., Fabio Lino ; Melo, Itamar Soares ^[6] ; Pizzirani-Kleiner, Aline Aparecida ^[7] ; van Elsas, Jan Dirk ^[8] ; Andreote, Fernando Dini ^[9]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo. Dept Soil Sci

^[2] Univ Sao Paulo. Dept Soil Sci

^[3] Univ Sao Paulo. Ctr Nucl Energy Agr

^[4] Univ Sao Paulo. Ctr Nucl Energy Agr

^[6] Embrapa Environm. Lab Environm Microbiol

^[7] Univ Sao Paulo. Dept Genet

^[8] Univ Groningen RUG. Dept Microbial Ecol

^[9] Univ Sao Paulo. Dept Soil Sci

• Tipo de documento: Artigo Científico
• Fonte: WATER AIR AND SOIL POLLUTION; v. 223, n. 7, p. 4233-4243, SEP 2012.
• Citações Web of Science: 12

Resumo

Mangrove ecosystems are tropical environments that are characterized by the interaction between the land and the sea. As such, this ecosystem is vulnerable to oil spills. Here, we show a culture-independent survey of fungal communities that are found in the sediments of the following two mangroves that are located on the coast of Sao Paulo State (Brazil): (1) an oil-spill-affected mangrove and (2) a nearby unaffected mangrove. Samples were collected from each mangrove forest at three distinct locations (transect from sea to land), and the samples were analyzed by quantitative PCR and internal transcribed spacer (ITS)-based PCR-DGGE analysis. The abundance of fungi was found to be higher in the oil-affected mangrove. Visual observation and correspondence analysis (CA) of the ITS-based PCR-DGGE profiles revealed differences in the fungal communities between the sampled areas. Remarkably, the oil-spilled area was quite distinct from the unaffected sampling areas. On the basis of the ITS sequences, fungi that are associated with the Basidiomycota and Ascomycota taxa were most common and belonged primarily to the genera Epicoccum, Nigrospora, and Cladosporium. Moreover, the Nigrospora fungal species were shown to be sensitive to oil, whereas a group that was described as ``uncultured Basidiomycota{''} was found more frequently in oil-contaminated areas. Our results showed an increase in fungal abundance in the oil-polluted mangrove regions, and these data indicated potential fungal candidates for remediation of the oil-affected mangroves. (AU)

• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 08/54013-8 - Diversidade de arquéias e bactérias envolvidas na ciclagem do nitrogênio em sedimentos de manguezais
• Beneficiário: Armando Cavalcante Franco Dias
• Modalidade de apoio: Bolsas no Brasil - Doutorado