Synergistic and additive effects of enriched environment and lithium on the generation of new cells in adult mouse hippocampus

Autor(es)

Schaeffer, Evelin L. ^[1] ; Cerulli, Fabiana G. ^[1] ; Souza, Helio O. X. ^[1] ; Catanozi, Sergio ^[2] ; Gattaz, Wagner F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept & Inst Psychiat, Fac Med, Lab Neurosci LIM 27, BR-05403010 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Div Endocrinol & Metab, Lipids Lab LIM 10, Clin Hosp, BR-01246000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEURAL TRANSMISSION; v. 121, n. 7, p. 695-706, JUL 2014.
• Citações Web of Science: 4

Resumo

Hippocampal atrophy is reported in several neuropathological disorders. The hippocampal dentate gyrus (DG) is a brain region where adult neurogenesis constitutively occurs. There are some reports suggesting the ability of endogenous neurogenesis to initiate neuronal repair in the hippocampus in response to neuropathological conditions, but its capacity to compensate for neuronal loss is limited. Among strategies to enhance adult hippocampal neurogenesis are enriched environment and lithium. This study aimed to assess whether both strategies could interact to potentiate the generation of new cells in the adult DG. Healthy adult male C57BL/6 mice were divided into four treatment groups for 28 days: control, lithium, enriched environment, enriched environment plus lithium. The animals were injected with BrdU (cell proliferation marker) shortly before the start of the treatments and killed 28 days later for analysis of newly generated cells. Two-way ANOVA followed by post hoc test revealed a significant synergistic interaction between enriched environment and lithium in the total number of BrdU(+) cells in the entire DG (p = 0.019), a trend towards significant synergistic interaction in the dorsal DG (p = 0.075), and a significant additive effect in the ventral DG (p = 0.001). These findings indicate that the combination of enriched environment and lithium has both synergistic and additive effects on the generation of new cells in the healthy adult DG (these effects being possibly segregated along the dorso-ventral axis of the hippocampus), and suggest that it might be worth investigating whether this combination would have a similar effect in neuropathological conditions. (AU)

• Processo FAPESP: 11/24022-8 - Efeito combinado de lítio e enriquecimento ambiental sobre a sobrevivência de novas células no hipocampo de camundongos adultos
• Beneficiário: Fabiana Gadotti Cerulli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/52825-8 - Neurobiologia da doença de Alzheimer: marcadores de risco, prognóstico e resposta terapêutica
• Beneficiário: Wagner Farid Gattaz
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/53008-3 - Efeitos da inibição crônica da fosfolipase A2 sobre a diferenciação de novos neurônios em células maduras no cérebro de ratos adultos
• Beneficiário: Evelin Lisete Schaeffer
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/23039-4 - Efeito combinado de lítio e enriquecimento ambiental sobre a sobrevivência de novas células no hipocampo de camundongos adultos
• Beneficiário: Hélio Oliveira Ximenes de Souza
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica