Interaction between mesenchymal stem cells and Ti-30Ta alloy after surface treatment

Autor(es)

Capellato, Patricia ^[1] ; Escada, Ana L. A. ^[2] ; Popat, Ketul C. ^[3] ; Alves Claro, Ana P. R. ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP. Fac Engn Guaratingueta

^[2] Sao Paulo State Univ UNESP. Fac Engn Guaratingueta

^[3] Colorado State Univ. Dept Mech Engn

^[4] Sao Paulo State Univ UNESP. Fac Engn Guaratingueta

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Materials Research Part A; v. 102, n. 7, p. 2147-2156, JUL 2014.
• Citações Web of Science: 0

Resumo

In this study, in vitro cytocompatibility was investigated in the Ti-30Ta alloy after two kinds of surfaces treatments: alkaline and biomimetic treatment. Each condition was evaluated by scanning electron microscopy/energy-dispersive X-ray spectroscopy. Cellular adhesion, viability, protein expression, morphology, and differentiation were evaluated with Bone marrow stromal cells (MSCs) to investigate the short and long-term cellular response by fluorescence microscope imaging and colorimetric assays techniques. Two treatments exhibited similar results with respect to total protein content and enzyme activity as compared with alloy without treatment. However, it was observed improved of the biomineralization, bone matrix formation, enzyme activity, and MSCs functionality after biomimetic treatment. These results indicate that the biomimetic surface treatment has a high potential for enhanced osseointegration. (C) 2013 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 10/07231-0 - Estudo in vitro e in vivo de implantes dentários da liga experimental Ti-30Ta após tratamento de superfície biomimético
• Beneficiário: Ana Paula Rosifini Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular