Natural Products from Cyanobacteria with Antimicrobial and Antitumor Activity

Autor(es)

Silva-Stenico, Maria Estela ^[1] ; Kaneno, Ramon ^[2] ; Zambuzi, Fabiana Albani ^[3] ; Vaz, Marcelo G. M. V. ^[4] ; Alvarenga, Danillo O. ^[5] ; Fiore, Marli Fatima ^[6]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo. Ctr Nucl Energy Agr

^[2] Sao Paulo State Univ. Dept Microbiol & Immunol

^[3] Sao Paulo State Univ. Dept Microbiol & Immunol

^[4] Univ Sao Paulo. Ctr Nucl Energy Agr

^[5] Univ Sao Paulo. Ctr Nucl Energy Agr

^[6] Univ Sao Paulo. Ctr Nucl Energy Agr

• Tipo de documento: Artigo Científico
• Fonte: CURRENT PHARMACEUTICAL BIOTECHNOLOGY; v. 14, n. 9, p. 820-828, OCT 2013.
• Citações Web of Science: 10

Resumo

Cyanobacteria are an important source of structurally bioactive metabolites, with cytotoxic, antiviral, anticancer, antimitotic, antimicrobial, specific enzyme inhibition and immunosuppressive activities. This study focused on the antitumor and antimicrobial activities of intra and extracellular cyanobacterial extracts. A total of 411 cyanobacterial strains were screened for antimicrobial activity using a subset of pathogenic bacteria as target. The in vitro antitumor assays were performed with extracts of 24 strains tested against two murine cancer cell lines (colon carcinoma CT-26 and lung cancer 3LL). Intracellular extracts inhibited 49 and 35% of Gram-negative and Gram-positive pathogenic bacterial growth, respectively. Furthermore, the methanolic intracellular extract of Cylindrospermopsis raciborskii CYP011K and Nostoc sp. CENA69 showed inhibitory activity against the cancer cell lines. The extracellular extract from Fischerella sp. CENA213 and M. aeruginosa NPJB-1 exhibited inhibitory activity against 3LL lung cancer cells at 0.8 mu g ml(-1) and Oxynema sp. CENA135, Cyanobium sp. CENA154, M. aeruginosa NPJB-1 and M. aeruginosa NPLJ-4 presented inhibitory activity against CT26 colon cancer cells at 0.8 mu g ml(-1). Other extracts were able to inhibit 3LL cell-growth at higher concentrations (20 mu g ml(-1)) such as Nostoc sp. CENA67, Cyanobium sp. CENA154 and M. aeruginosa NPLJ-4, while CT26 cells were inhibited at the same concentration by Nostoc sp. CENA67 and Fischerella sp. CENA213. These extracts presented very low inhibitory activity on human peripheral blood lymphocytes. The results showed that some cyanobacterial strains are a rich source of natural products with potential for pharmacological and biotechnological applications. (AU)

• Processo FAPESP: 09/05474-5 - Bioprospecção de cianobactéria: seleção de cianopeptídeos para aplicação farmacêutica e biotecnológica
• Beneficiário: Maria Estela Stenico
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/18732-0 - Produtos naturais bioativos de cianobactérias marinhas
• Beneficiário: Marcelo Gomes Marçal Vieira Vaz
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/20307-8 - Efeito de baixas concentrações de agentes antineoplásicos sobre linfócitos humanos de sangue periférico
• Beneficiário: Fabiana Albani Zambuzi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/08092-6 - Sequenciamento do genoma da cianobactéria Nostoc sp. CENA67 e anotação de genes do metabolismo secundário
• Beneficiário: Danillo Oliveira de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/09867-9 - Bioprospecção de cianobactéria: seleção de cianopeptídeos para aplicação farmacêutica e biotecnológica
• Beneficiário: Maria Estela Stenico
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores