| Texto completo | |
| Autor(es): Mostrar menos - |
Tribuiani, Natalia
[1]
;
da Silva, Alexandro Mateus
[2]
;
Ferraz, Miriele Cristina
[3]
;
Silva, Magali Glauzer
[4]
;
Guerreiro Bentes, Ana Paula
[5]
;
Graziano, Talita Signoreti
[6]
;
dos Santos, Marcio Galdino
[7]
;
Cogo, Jose Carlos
[8]
;
Varanda, Eliana Aparecida
[9]
;
Groppo, Francisco Carlos
[10]
;
Cogo, Karina
[11]
;
Oshima-Franco, Yoko
[12]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): Mostrar menos - | [1] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[2] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[3] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[4] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[5] Univ Campinas UNICAMP. Piracicaba Dent Sch
[6] Univ Campinas UNICAMP. Piracicaba Dent Sch
[7] Tocantins Fed Univ UFT. Environm Sci Postgrad Program
[8] Serpentarium Vale Paraiba Univ CEN UNIVAP. BR-12244000 Sao Jose Dos Campos
[9] Sao Paulo State Univ UNESP. Pharmaceut Sci Fac Araraquara
[10] Univ Campinas UNICAMP. Piracicaba Dent Sch
[11] Univ Campinas UNICAMP. Piracicaba Dent Sch
[12] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
Número total de Afiliações: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; v. 14, FEB 8 2014. |
| Citações Web of Science: | 7 |
| Resumo | |
Background: Snakebite is a significant public health issue in tropical countries. In Brazil, some of the most common snake envenomations are from Bothrops. Bothrops bites trigger local and systemic effects including edema, pain, erythema, cyanosis, infections, and necrosis. Vellozia flavicans is a plant from the Brazilian ``cerrado{''} (savanna) that is popularly used as an anti-inflammatory medicine. Since inflammation develops quickly after Bothrops bites, which can lead to infection, the aim of the present study was to observe possible anti-snake venom and antimicrobial activities of V. flavicans (Vf). Methods: The chromatographic profile of the main constituents from the Vf leaf hydroalcoholic extract was obtained by thin-layer chromatography (TLC). The anti-snake venom activity was measured by Vf's ability to neutralize the in vitro neuromuscular blockade caused by Bothrops jararacussu venom (Bjssu) in a mouse phrenic nerve-diaphragm model (PND). After a 20 min incubation, preparations of PND were added to Tyrode's solution (control); Vf (0.2, 0.5, 1, and 2 mg/mL); 40 mu g/mL Bjssu; pre-incubation for 30 min with Bjssu and 1 mg/mL Vf; and a Bjssu pretreated preparation (for 10 min) followed by 1 mg/mL Vf. Myographic recording was performed, and the contractile responses were recorded. The antimicrobial activity (minimum inhibitory concentration {[}MIC] and minimum bactericidal concentration {[}MBC]) was obtained for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, using gentamicin and vancomycin as positive controls. Results: TLC analysis yielded several compounds from Vf, such as flavonoids (quercetin) and phenolic acids (chlorogenic acid). Bjssu completely blocked the contractile responses of PND preparations, while Vf preserved 97% (+/- 10%) of the contractile responses when incubated with Bjssu. In the PND pretreated with Bjssu, Vf was able to inhibit the neuromuscular blockade progress. MIC and MBC of Vf ranged from 2.5 to 5.0 mg/mL for P. aeruginosa and S. aureus strains, while no antimicrobial activity was observed for E. coli and E. faecalis. Conclusions: The hydroalcoholic extract from Vf leaves was able to neutralize and decrease the in vitro neuromuscular blockade caused by Bjssu. However, it did not show significant antimicrobial activity against the tested bacteria. (AU) | |
| Processo FAPESP: | 08/11005-5 - Dipteryx alata Vogel: estudos da ação antiofídica e de toxicidade |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Bolsas no Exterior - Pesquisa |
| Processo FAPESP: | 04/09705-8 - Investigacao farmacologica da atividade antipeconhenta de fracoes obtidas de extratos de casearia sylvestris sw. |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/08271-0 - IDENTIFICAÇÃO E FARMACODINÂMICA DE FRAÇÕES NEUROFACILITADORAS DA Casearia sylvestris SW. NA JUNÇÃO NEUROMUSCULAR |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 08/52643-4 - Estudo da capacidade neutralizante do extrato hidroalcoolico de camellia sinensis (cha verde) contra os efeitos neurotoxico e miotoxico da bothropstoxina-i (bothrops jararacussu). |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 07/53883-6 - Dipteryx alata vogel: estudo da acao antiofidica e da toxicidade. |
| Beneficiário: | Yoko Oshima Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |