| Full text | |
| Author(s): Show less - |
Tribuiani, Natalia
[1]
;
da Silva, Alexandro Mateus
[2]
;
Ferraz, Miriele Cristina
[3]
;
Silva, Magali Glauzer
[4]
;
Guerreiro Bentes, Ana Paula
[5]
;
Graziano, Talita Signoreti
[6]
;
dos Santos, Marcio Galdino
[7]
;
Cogo, Jose Carlos
[8]
;
Varanda, Eliana Aparecida
[9]
;
Groppo, Francisco Carlos
[10]
;
Cogo, Karina
[11]
;
Oshima-Franco, Yoko
[12]
Total Authors: 12
|
| Affiliation: Show less - | [1] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[2] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[3] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[4] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
[5] Univ Campinas UNICAMP. Piracicaba Dent Sch
[6] Univ Campinas UNICAMP. Piracicaba Dent Sch
[7] Tocantins Fed Univ UFT. Environm Sci Postgrad Program
[8] Serpentarium Vale Paraiba Univ CEN UNIVAP. BR-12244000 Sao Jose Dos Campos
[9] Sao Paulo State Univ UNESP. Pharmaceut Sci Fac Araraquara
[10] Univ Campinas UNICAMP. Piracicaba Dent Sch
[11] Univ Campinas UNICAMP. Piracicaba Dent Sch
[12] Univ Sorocaba UNISO. Pharmaceut Sci Postgrad Program
Total Affiliations: 12
|
| Document type: | Journal article |
| Source: | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE; v. 14, FEB 8 2014. |
| Web of Science Citations: | 7 |
| Abstract | |
Background: Snakebite is a significant public health issue in tropical countries. In Brazil, some of the most common snake envenomations are from Bothrops. Bothrops bites trigger local and systemic effects including edema, pain, erythema, cyanosis, infections, and necrosis. Vellozia flavicans is a plant from the Brazilian ``cerrado{''} (savanna) that is popularly used as an anti-inflammatory medicine. Since inflammation develops quickly after Bothrops bites, which can lead to infection, the aim of the present study was to observe possible anti-snake venom and antimicrobial activities of V. flavicans (Vf). Methods: The chromatographic profile of the main constituents from the Vf leaf hydroalcoholic extract was obtained by thin-layer chromatography (TLC). The anti-snake venom activity was measured by Vf's ability to neutralize the in vitro neuromuscular blockade caused by Bothrops jararacussu venom (Bjssu) in a mouse phrenic nerve-diaphragm model (PND). After a 20 min incubation, preparations of PND were added to Tyrode's solution (control); Vf (0.2, 0.5, 1, and 2 mg/mL); 40 mu g/mL Bjssu; pre-incubation for 30 min with Bjssu and 1 mg/mL Vf; and a Bjssu pretreated preparation (for 10 min) followed by 1 mg/mL Vf. Myographic recording was performed, and the contractile responses were recorded. The antimicrobial activity (minimum inhibitory concentration {[}MIC] and minimum bactericidal concentration {[}MBC]) was obtained for Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Enterococcus faecalis, using gentamicin and vancomycin as positive controls. Results: TLC analysis yielded several compounds from Vf, such as flavonoids (quercetin) and phenolic acids (chlorogenic acid). Bjssu completely blocked the contractile responses of PND preparations, while Vf preserved 97% (+/- 10%) of the contractile responses when incubated with Bjssu. In the PND pretreated with Bjssu, Vf was able to inhibit the neuromuscular blockade progress. MIC and MBC of Vf ranged from 2.5 to 5.0 mg/mL for P. aeruginosa and S. aureus strains, while no antimicrobial activity was observed for E. coli and E. faecalis. Conclusions: The hydroalcoholic extract from Vf leaves was able to neutralize and decrease the in vitro neuromuscular blockade caused by Bjssu. However, it did not show significant antimicrobial activity against the tested bacteria. (AU) | |
| FAPESP's process: | 08/11005-5 - Dipteryx alata Vogel: antiophidian action and toxicity |
| Grantee: | Yoko Oshima Franco |
| Support Opportunities: | Scholarships abroad - Research |
| FAPESP's process: | 12/08271-0 - IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION |
| Grantee: | Yoko Oshima Franco |
| Support Opportunities: | Regular Research Grants |