IDENTIFICATION AND PHARMACODYNAMIC OF NEUROFACILITATING FRACTIONS OF Casearia sylvestris SW. AT THE NEUROMUSCULAR JUNCTION

• Grant number: 12/08271-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Pharmacology - Autonomic Pharmacology
• Principal Investigator: Yoko Oshima Franco
• Grantee: Yoko Oshima Franco
• Host Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
• Associated researchers: Renata Vasques da Silva Tavares

Abstract

Although several medicinal potentialities have been described to essential oil and to extracts from distinct parts of Casearia sylvestris plant, and mainly due its toxicity restrictions have been described in specific situations, our objectives are to identify pharmacologically the facilitatory effect in different organic fractions of C. sylvestris leaves, as well as the pharmacodynamic of this effect. Previous studies showed that either aqueous as hydroalcoholic extracts induced a dose-dependent facilitatory effect using mouse nerve phrenic-diaphragm preparation. Thus, the fractioning of C. sylvestris hydroalcoholic extract it will permit to identify which of fractions will exhibit such pharmacological effect aiming the further pharmacodynamic study. The clinical benefits in the search of new facilitatory agents are extensive. Depending on its pharmacodynamic, under neuromuscular junction parameter, it is possible to think in applying to the neuromuscular diseases that induce paralysis, as myastenic syndromes and Myastenia gravis; as an adjuvant in cases of botulin toxin or tetrodotoxin envenomation; as neuromuscular recovers after surgery controlled by the non depolarizing drugs; in chronic obstructive pulmonary diseases, as some examples. Whether these objectives were reached with a natural compound and using a plant which has been extensively studied under several approaches as Casearia sylvestris, the scientific gain will be major. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TRIBUIANI, NATALIA; DA SILVA, ALEXANDRO MATEUS; FERRAZ, MIRIELE CRISTINA; SILVA, MAGALI GLAUZER; GUERREIRO BENTES, ANA PAULA; GRAZIANO, TALITA SIGNORETI; DOS SANTOS, MARCIO GALDINO; COGO, JOSE CARLOS; VARANDA, ELIANA APARECIDA; GROPPO, FRANCISCO CARLOS; et al. Vellozia flavicans Mart. ex Schult. hydroalcoholic extract inhibits the neuromuscular blockade induced by Bothrops jararacussu venom. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 14, FEB 8 2014. (08/11005-5, 12/08271-0)

YOSHIDA, EDSON H.; TRIBUIANI, NATALIA; FORAMIGLIO, AMERIS L.; FORAMIGLIO, CAROLINE A.; DA SILVA TAVARES, RENATA V.; BONOMINI, TIAGO J.; BUENO, PAULA C. P.; CAVALHEIRO, ALBERTO J.; HYSLOP, STEPHEN; PUEBLA, PILAR; et al. A Highly Polar Phytocomplex Involving Rutin is Responsible for the Neuromuscular Facilitation Caused by Casearia sylvestris (guacatonga). CURRENT PHARMACEUTICAL BIOTECHNOLOGY, v. 17, n. 15, p. 1360-1368, 2016. (08/11005-5, 12/08271-0)

ROSTELATO-FERREIRA, SANDRO; VETTORAZZO, ORLANDO B.; TRIBUIANI, NATALIA; LEAL, ALLAN P.; DAL BELO, CHARISTON A.; RODRIGUES-SIMIONI, LEA; FLORIANO, RAFAEL S.; OSHIMA-FRANCO, YOKO. Action of heparin and acetylcholine modulators on the neurotoxicity of the toad Rhinella schneideri (Anura: Bufonidae) in Brazil. Revista de Biología Tropical, v. 69, n. 2, p. 545-556, APR-JUN 2021. (12/08271-0)

FONTANA OLIVEIRA, ISADORA CARUSO; DE PAULA, MARINA OLIVEIRA; BOSCHILHA LASTRA, HELLEN CRISTINE; ALVES, BRUNO DE BRITO; NETO MORENO, DEBORA ANTUNES; YOSHIDA, EDSON HIDEAKI; AMARAL FILHO, JORGE; COGO, JOSE CARLOS; VARANDA, ELIANA APARECIDA; RAI, MAHENDRA; et al. Activity of silver nanoparticles on prokaryotic cells and Bothrops jararacussu snake venom. DRUG AND CHEMICAL TOXICOLOGY, v. 42, n. 1, p. 60-64, JAN 2 2019. (08/11005-5, 15/01420-9, 12/08271-0)

HARDER, CAROLINA; DE OLIVEIRA, AKILA LARA; SCRIBONI, ANDREIA BORGES; OLIVEIRA CINTRA, ADELIA CRISTINA; SCHEZARO-RAMOS, RAPHAEL; DOS SANTOS, MARCIO GALDINO; COGO-MUELLER, KARINA; HASHIMOTO MIURA, REGINA YURI; FLORIANO, RAFAEL STUANI; ROSTELATO-FERREIRA, SANDRO; et al. Pharmacological Properties of Vochysia Haenkeana (Vochysiaceae) Extract to Neutralize the Neuromuscular Blockade Induced by Bothropstoxin-I (Lys49 Phospholipase A(2)) Myotoxin. ADVANCED PHARMACEUTICAL BULLETIN, v. 7, n. 3, p. 433-439, SEP 2017. (15/01420-9, 12/08271-0)

PROENCA-ASSUNCAO, JAQUELINE DE CASSIA; CONSTANTINO, EDERSON; FARIAS-DE-FRANCA, ANNA PAULA; NOGUEIRA, FLAVIA APARECIDA RESENDE; CONSONNI, SILVIO ROBERTO; CHAUD, MARCO VINICIUS; DOS SANTOS, CAROLINA ALVES; OSHIMA-FRANCO, YOKO. Mutagenicity of silver nanoparticles synthesized with curcumin (Cur-AgNPs). JOURNAL OF SAUDI CHEMICAL SOCIETY, v. 25, n. 9, SEP 2021. (19/05891-7, 12/08271-0)

PROENCA-ASSUNCAO, JAQUELINE DE CASSIA; FARIAS-DE-FRANCA, ANNA PAULA; TRIBUIANI, NATALIA; COGO, JOSE CARLOS; COLLACO, RITA DE CASSIA; RANDAZZO-MOURA, PRISCILA; CONSONNI, SILVIO ROBERTO; CHAUD, MARCO VINICIUS; DOS SANTOS, CAROLINA ALVES; OSHIMA-FRANCO, YOKO. The Influence of Silver Nanoparticles Against Toxic Effects of Philodryas olfersii Venom. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 16, p. 3555-3564, 2021. (12/08271-0, 19/05891-7)

DOS SANTOS, MARCIO G.; MORENO, FELIPE S.; DE OLIVEIRA, AKILA L.; OLIVEIRA, ISADORA C. F.; TRIBUIANI, NATALIA; COGO, JOSE C.; SCRIBONI, ANDREIA B.; COGO-MUELLER, KARINA; SAN FELICIANO, ARTURO; BONOMINI, TIAGO JOSE; et al. New pharmacological insights of Galactia glaucescens. NATURAL PRODUCT RESEARCH, v. 33, n. 16, p. 2389-2393, AUG 18 2019. (14/26786-3, 15/01420-9, 12/08271-0)

FONTANA OLIVEIRA, ISADORA CARUSO; DALLA MORA, ADRIANA; NETO, JAIRO BRAGA; DE OLIVEIRA, AKILA LARA; HARDER, CAROLINA; HANAI-YOSHIDA, VALQUIRIA MIWA; COGO, JOSE CARLOS; FLORIANO, RAFAEL STUANI; BONVENTI JUNIOR, WALDEMAR; OLIVEIRA, SARA DE JESUS; et al. Prospective study of a Bothrops jararacussu venom batch (Bj2015) - phospholipase A(2) activity, immunogenicity, neurotoxicity, and myotoxicity parameters. NATURAL PRODUCT RESEARCH, v. 33, n. 16, p. 2417-2421, AUG 18 2019. (15/01420-9, 12/08271-0)

TRIBUIANI, NATALIA; TAVARES, MARYLU OLIVEIRA; SANTANA, MONIQUE NEVES; FONTANA OLIVEIRA, ISADORA CARUSO; DO AMARAL FILHO, JORGE; SILVA, MAGALI GLAUZER; DOS SANTOS, MARCIO GALDINO; COGO, JOSE CARLOS; FLORIANO, RAFAEL STUANI; COGO-MULLER, KARINA; et al. Neutralising ability of Terminalia fagifolia extract (Combretaceae) against the in vitro neuromuscular effects of Bothrops jararacussu venom. NATURAL PRODUCT RESEARCH, v. 31, n. 23, p. 2783-2787, 2017. (12/08271-0)

YOSHIDA, EDSON HIDEAKI; TRIBUIANI, NATALIA; SABADIM, GIOVANA; NETO MORENO, DEBORA ANTUNES; VARANDA, ELIANA APARECIDA; OSHIMA-FRANCO, YOKO. Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test. ADVANCED PHARMACEUTICAL BULLETIN, v. 6, n. 3, p. 443-447, SEP 2016. (08/11005-5, 12/08271-0)

WERNER, ADRIANA C.; FERRAZ, MIRIELE C.; YOSHIDA, EDSON H.; TRIBUIANI, NATALIA; GAUTUZ, JEAN A. A.; SANTANA, MONIQUE N.; DEZZOTTI, BRUNA A.; DE MIRANDA, VANESSA G.; FORAMIGLIO, AMERIS L.; ROSTELATO-FERREIRA, SANDRO; et al. The Facilitatory Effect of Casearia sylvestris Sw. (guacatonga) Fractions on the Contractile Activity of Mammalian and Avian Neuromuscular Apparatus. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, v. 16, n. 5, p. 468-481, 2015. (08/11005-5, 12/08271-0)

NETO MORENO, DEBORA ANTUNES; SALADIN, MIRELA SANTOS; MARQUES VIROEL, FABIA JUDICE; JUSTE DINI, MURILO MELO; PICKIER, THAISA BORIM; AMARAL FILHO, JORGE; DOS SANTOS, CAROLINA ALVES; HANAI-YOSHIDA, VALQUIRIA MIWA; GROTTO, DENISE; GERENUTTI, MARLI; et al. Are Silver Nanoparticles Useful for Treating Second-Degree Burns? An Experimental Study in Rats. ADVANCED PHARMACEUTICAL BULLETIN, v. 11, n. 1, p. 130-136, 2021. (12/08271-0, 15/01420-9, 08/11005-5)

VETTORAZZO, ORLANDO BOZZONI; TRIBUIANI, NATALIA; SILVA, FANI RIBEIRO; ROSTELATO-FERREIRA, SANDRO; OSHIMA-FRANCO, YOKO. HEPARIN AT A LOW CONCENTRATION AGAINST THE NEUROMUSCULAR BLOCKING EFFECT INDUCED BY THE RHINELLA SCHNEIDERI VENOM. Toxicon, v. 168, p. 1-pg., 2019-10-05. (12/08271-0)