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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Sequence and expression pattern of the germ line marker vasa in honey bees and stingless bees

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Autor(es):
Tanaka, Erica Donato [1, 2] ; Hartfelder, Klaus [3]
Número total de Autores: 2
Afiliação do(s) autor(es):
[1] Univ Pompeu Fabra, Consejo Super Invest Cient, Inst Biol Evolut, E-08003 Barcelona - Spain
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14049 Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol Bioagentes Patogen, Ribeirao Preto - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 32, n. 3, p. 582-U384, 2009.
Citações Web of Science: 7
Resumo

Queens and workers of social insects differ in the rates of egg laying. Using genomic information we determined the sequence of vasa, a highly conserved gene specific to the germ line of metazoans, for the honey bee and four stingless bees. The vasa sequence of social bees differed from that of other insects in two motifs. By RT-PCR we confirmed the germ line specificity of Amvasa expression in honey bees. In situ hybridization on ovarioles showed that Amvasa is expressed throughout the germarium, except for the transition zone beneath the terminal filament. A diffuse vasa signal was also seen in terminal filaments suggesting the presence of germ line cells. Oocytes showed elevated levels of Amvasa transcripts in the lower germarium and after follicles became segregated. In previtellogenic follicles, Amvasa transcription was detected in the trophocytes, which appear to supply its mRNA to the growing oocyte. A similar picture was obtained for ovarioles of the stingless bee Melipona quadrifasciata, except that Amvasa expression was higher in the oocytes of previtellogenic follicles. The social bees differ in this respect from Drosophila, the model system for insect oogenesis, suggesting that changes in the sequence and expression pattern of vasa may have occurred during social evolution. (AU)

Processo FAPESP: 99/00719-6 - Abelhas africanizadas: análise integrada do processo de africanização de Apis mellifera com foco sobre determinantes da fertilidade de zangões, rainhas e operárias
Beneficiário:Zilá Luz Paulino Simões
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 02/11729-7 - Sequenciamento e expressão do gene vasa em Apis mellifera: um marcador da linhagem germinativa e o estudo comparado da ovogênese em Melipona quadrifasciata
Beneficiário:Erica Donato Tanaka
Linha de fomento: Bolsas no Brasil - Doutorado