Preparation, Characterization, Cytotoxicity, and Genotoxicity Evaluations of Thiolated- and S-Nitrosated Superparamagnetic Iron Oxide Nanoparticles: Implications for Cancer Treatment

Autor(es)

Seabra, Amedea B. ^[1] ; Pasquoto, Tatiane ^[2] ; Ferrarini, Ana Carolina F. ^[1] ; Santos, Marconi da Cruz ^[1] ; Haddad, Paula S. ^[1] ; de Lima, Renata ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Exact & Earth Sci Dept, BR-09913030 Diadema, SP - Brazil

^[2] Univ Fed Sao Carlos, UFSCar, BR-18052780 Sao Paulo - Brazil

^[3] Univ Sorocaba, Dept Biotechnol, BR-18023000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemical Research in Toxicology; v. 27, n. 7, p. 1207-1218, JUL 2014.
• Citações Web of Science: 32

Resumo

Iron oxide magnetic nanoparticles have been proposed for an increasing number of biomedical applications, such as drug delivery. To this end, toxicological studies of their potent effects in biological media must be better evaluated. The aim of this study was to synthesize, characterize, and examine the potential in vitro cytotoxicity and genotoxicity of thiolated (SH) and S-nitrosated (S-NO) iron oxide superparamagnetic nanoparticles toward healthy and cancer cell lines. Fe3O4 nanoparticles were synthesized by coprecipitation techniques and coated with small thiol-containing molecules, such as mercaptosuccinic acid (MSA) or meso-2,3-dimercaptosuccinic acid (DMSA). The physical chemical, morphological, and magnetic properties of thiol-coating Fe3O4 nanoparticles were characterized by different techniques. The thiol groups on the surface of the nanoparticles were nitrosated, leading to the formation of S-nitroso-MSA- or S-nitroso-DMSA-Fe3O4 nanoparticles. The cytotoxicity and genotoxicity of thiolated and S-nitrosated nanoparticles were more deeply evaluated in healthy (3T3, human lymphocytes cells, and chinese hamster ovary cells) and cancer cell lines (MCF-7). The results demonstrated that thiol-coating iron oxide magnetic nanoparticles have few toxic effects in cells, whereas S-nitrosated-coated particles did cause toxic effects. Moreover, due to the superaramagnetic behavior of S-nitroso-Fe3O4 nanopartides, those particles can be guided to the target site upon the application of an external magnetic field, leading to local toxic effects in the tumor cells. Taken together, the results suggest the promise of S-nitroso-mgnetic nanopartides in cancer treatment. (AU)

• Processo FAPESP: 11/10125-0 - Aplicações biomédicas de nanopartículas magnéticas
• Beneficiário: Paula Silvia Haddad Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17053-7 - Nanopartículas doadoras de óxido nítrico para aplicações biomédicas
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular