| Texto completo | |
| Autor(es): |
Aissa, Alexandre Ferro
[1]
;
Tryndyak, Volodymyr
[2]
;
de Conti, Aline
[2]
;
Melnyk, Stepan
[3]
;
Ursula Hermogenes Gomes, Tarsila Daysy
[4]
;
Pires Bianchi, Maria Lourdes
[4]
;
James, S. Jill
[3]
;
Beland, Frederick A.
[2]
;
Greggi Antunes, Lusania Maria
[4, 1]
;
Pogribny, Igor P.
[2]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Sao Paulo - Brazil
[2] US FDA, Div Biochem Toxicol, NCTR, Jefferson, AR 72079 - USA
[3] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 - USA
[4] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MOLECULAR NUTRITION & FOOD RESEARCH; v. 58, n. 7, p. 1502-1512, JUL 2014. |
| Citações Web of Science: | 15 |
| Resumo | |
Scope: A compromised nutritional status in methyl-group donors may provoke several molecular alterations triggering the development of nonalcoholic fatty liver disease (NAFLD) in humans and experimental animals. In this study, we investigated a role and the underlying molecular mechanisms of methionine metabolic pathway malfunctions in the pathogenesis of NAFLD. Methods and results: We fed female Swiss albino mice a control (methionine-adequate) diet and two experimental (methionine-deficient or methionine-supplemented) diets for 10 weeks, and the levels of one-carbon metabolites, expression of one-carbon and lipid metabolism genes in the livers were evaluated. We demonstrate that both experimental diets increased hepatic levels of S-adenosyl-L-homocysteine and homocysteine, altered expression of one-carbon and lipid metabolism genes, and caused lipid accumulation, especially in mice fed the methionine-deficient diet. Markers of oxidative and ER stress response were also elevated in the livers of mice fed either diet. Conclusion: Our findings indicate that both dietary methionine deficiency and methionine supplementation can induce molecular abnormalities in the liver associated with the development of NAFLD, including deregulation in lipid and one-carbon metabolic pathways, and induction of oxidative and ER stress. These pathophysiological events may ultimately lead to lipid accumulation in the livers, triggering the development of NAFLD. (AU) | |
| Processo FAPESP: | 10/01410-0 - Interação gene-dieta em camundongos suplementados com metionina nos períodos fetal e pós-natal: estudos de instabilidade genômica, metilação e expressão de genes relacionados com doenças cardiovasculares |
| Beneficiário: | Alexandre Ferro Aissa |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/10872-2 - Análise da expressão de microRNAs em camundongos fêmeas alimentadas com dietas deficiente ou suplementada em metionina durante a prenhez |
| Beneficiário: | Alexandre Ferro Aissa |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |